LOCUS       sequence-81431-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast plasmid for integration of target sequence at HO locus, Kan 
            selection, pMET inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: HO-pMET-poly-KanMX4-HO
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(616..813)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ctatagggcg aattggggat cgatccacta gttctagcta gagcggccgc ataggccact
       61 gtaagattcc gccacatttt atacactctg gtcctttaac tggcaaacct tcgggcgtaa
      121 tgcccaattt ttcgcctttg tcttttgcct ttttcacttc acgtgcttct ggtacatact
      181 tgcaatttat acagtgatga ccgctgaatt tgtatcttcc atagcatcta gcacatactc
      241 gatttttacc actccaatct ttataaaaat acttgattcc ctttctggga caagcaacac
      301 agtgttttag attctttttt tgtgatattt tatgctgttc tcccacacag cagcctcgac
      361 atgatttcac ttctattttg ttgccaagca agaaattttt atggccttct atcgtaagcc
      421 catatacagt actctcaccc tggaaatcat ccgtgaagct gaaatatacg ggttcccttt
      481 ttataattgg cggaacttct cttgttttgt gaccacttcg acaatatgac aaaacattct
      541 gtgaagttgt tcccccagga attaattcga gctcgttttc gacactggat ggcggcgtta
      601 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
      661 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      721 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      781 gttcttgaaa acaagaatct ttttattgtc agtactgatt agaaaaactc atcgagcatc
      841 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaa
//
