LOCUS       sequence-81434-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast plasmid for integration of target sequence at HO locus, Kan 
            selection, pMET inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: HO-pMET-poly-KanMX4-HO
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..47)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(31..47)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(48..397)
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     promoter        497..840
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(908..927)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagta tggatggggg
       61 taatagaatt gtatctatgt atctgacgac cctgtattac acgaaggaaa tagtagatga
      121 aaagacaaga gagcaagaaa aaggaaaaac ttcctttcta acagacgctt tacttaacct
      181 tatatatatc ttattttttt catcgagcgt gttcaattgg acaaggtgcc accttttcga
      241 cacttcggtt attatgttac acagttttca tgaggatggc gccttgacta acttaattag
      301 tcatttgcca accaccacag ttccccaata tcgacagctt cacgtgccat ttgccatttt
      361 gcgatcatgt gacctcaaga gaaaaagcaa aaaataatga gagctaaggg attcgaaccc
      421 ttgcatccgg aggggttaat taaggcgcgc cagatctgtt tagcttgcct cgtccccgcc
      481 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
      541 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
      601 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
      661 gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
      721 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
      781 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc
      841 atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat
      901 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      961 ttgtatggga agcccgatgc gccaga
//
