LOCUS       sequence-81493-e       11667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses I-SceI nuclease in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pKF257
FEATURES             Location/Qualifiers
     source          1..11667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..202,203..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(771..790)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     890..912
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(950..968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1036..1140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1141..1209,1210..2001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1359..1378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2172..2760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2661..2680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(2995..3025,3026..3580)
                     /label=P element 5' end
     gene            complement(3591..7719)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     7692..7710
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             10416..10436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10455..10481
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgcgtactcg caaattatta aaaataaaac tttaaaaata atttcgtcta attaatatta
       61 tgagttaatt caaaccccac ggacatgcta agggttaatc aacaatcata tcgctgtctc
      121 actcagactc aatacgacac tcagaatact attcctttca ctcgcactta ttgcaagcat
      181 acgttaagtg gatgtctctt gccgacggga ccaccttatg ttatttcatc atggtctggc
      241 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg
      301 caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac
      361 catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata
      421 aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga
      481 ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc
      541 gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc
      601 gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc
      661 cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag
      721 ttccgcgcga ttcggtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      781 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      841 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      901 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
      961 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1021 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1141 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1201 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1261 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1321 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1381 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1441 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1501 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1561 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1621 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1681 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1741 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1801 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1861 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1921 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1981 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2041 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2101 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2281 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2521 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     2581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2761 aacgccttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc
     2821 tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg
     2881 ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg
     2941 gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct ggcccatgat
     3001 gaaataacat aaggtggtcc cgtcgatagc cgaagcttac cgaagtatac acttaaattc
     3061 agtgcacgtt tgcttgttga gaggaaaggt tgtgtgcgga cgaatttttt tttgaaaaca
     3121 ttaaccctta cgtggaataa aaaaaaatga aatattgcaa attttgctgc aaagctgtga
     3181 ctggagtaaa attaattcac gtgccgaagt gtgctattaa gagaaaattg tgggagcaga
     3241 gccttgggtg cagccttggt gaaaactccc aaatttgtga tacccacttt aaagattcgc
     3301 agtggaaggc tgcacctgca aaaggtcaga catttaaaag gaggcgactc aacgcagatg
     3361 ccgtacctag taaagtgata gagcctgaac cagaaaagat aaaagaaggc tataccagtg
     3421 ggagtacaca aacagagtaa gtttgaatag taaaaaaaat catttatgta aacaataacg
     3481 tgactgtgcg ttaggtcctg ttcattgttt aatgaaaata agagcttgag ggaaaaaatt
     3541 cgtactttgg agtacgaaat gcgtcgttta gagcagcagc cgaattaatt ctagttccag
     3601 tgaaatccaa gcattttcta aattaaatgt attcttatta ttatagttgt tatttttgat
     3661 atatataaac aacactatta tgcccaccat ttttttgaga tgcatctaca caaggaacaa
     3721 acactggatg tcactttcag ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa
     3781 aattacctta aaaagtcata atattaaatt agaataaata tagctgtgag ggaaatatat
     3841 acaaatatat tggagcaaat aaattgtaca tacaaatatt tattactaat ttctattgag
     3901 acgaaatgaa ccactcggaa ccatttgagc gaaccgaatc gcgcggaact aacgacagtc
     3961 gctccaaggt cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa
     4021 agagcaacta cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa
     4081 aagtatgtat ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg
     4141 taaggtatgc aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg
     4201 gatgttcgaa ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa
     4261 tataattaca ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta
     4321 aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa
     4381 gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt
     4441 agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac
     4501 tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt
     4561 ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga
     4621 ggtatactgg caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga
     4681 taaccggcgg acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat
     4741 atccgaagga cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt
     4801 gcagcactcc ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca
     4861 ctgtgagaaa aagcggtaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat
     4921 aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg
     4981 aatcgattca ttagaaagtt agtaaattat taatatgcaa atgtattcta aacaagactt
     5041 acatttatcg tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct
     5101 ccgttgatat tcatcacgcc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc
     5161 aagatggcaa ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcatatcttc
     5221 aacatgttcg cgagttaaat gaaatttatt tattttctgc aaaactataa actatacatc
     5281 tcattgaaaa aaactaagaa gggtgtggaa tcaggcaatt ctaactaaaa tctagcgaat
     5341 ttgtttccaa gaattgtaag cgttatatca tttgtttcca ctggaaccac tcaccgttgt
     5401 ctgaataagt cgcactttta cgaggagtgg ttccttgagc accgacagcc aggatcgcca
     5461 caggaccgcc cggaactgca tgaaccaggt ggccttgtag gtgtacccat tctccggctg
     5521 ctccagtggc ttctccaaat ttttggtggc caacaactgc tccatatccc gggctacttt
     5581 gctaatagca aaattgtcgc catatcttgg cgatccgatc acgggactcg atctcccgtc
     5641 cgggcacaac ggccaacacc tgtacgtaaa agtccgccgg attgtagttg gtaggacact
     5701 gggcacccac gctggatagg agttgagatg taatgtaatg ctagataccc ttaataaaca
     5761 catcgaactc actaggaaaa gaagtcgacg gcttcgctgg gagtgcccaa gaaagctacc
     5821 ctgccctcgg ccatcagaag gatcttgtca aagagctcaa acagctcgga agacggctga
     5881 tgaatggtca ggatgacggt cttgcccttc tgcgacagct tcttcagcac ctggacgacg
     5941 ctgtgggcgg taaatgagtc cagtccggag gtgggctcat cgcagatcag aagcggcgga
     6001 tcggttagtg cctcggaggc gaatgccaga cgcttccttt ctccgccgga cagacctttc
     6061 accctgccgg gcacaccgat gatcgtgtgc tgacatttgc tgagcgaaag ctcctggatc
     6121 acctgatcca cgcgggccac tcgctgccga taggtcagat gtcgtggcat ccgcaccatg
     6181 gcctggaaaa tcaggtgttc cctggccgtt agggagccga taaagaggtc atcctgctgg
     6241 acataggcgc acctggcctg catctccttg gcgtccacag gttggccatt gagcagtcgc
     6301 atcccggatg gcgatacttg gatgccctgc ggcgatcgaa aggcaagggc attcagcagg
     6361 gtcgtctttc cggcaccgga actgcccatc acggccaaaa gttcgcccgg ataggccacg
     6421 ccgcaaactg agtttcaaat tggtaattgg accctttatt aagatttcac acagatcagc
     6481 cgactgcgaa tagaaactca ccgttcttga gcaaatgttt cctgggcgcc ggtatgtgtc
     6541 gctcgttgca gaatagtccg cgtgtccggt tgaccagctg ccgccatccg gagcccggct
     6601 gattgaccgc cccaaagatg tccatattgt gccaggcata ggtgaggttc tcggctagtt
     6661 ggccgctccc tgaaccggag tcctccggcg gactgggtgg caggagcgtg ccgtagtttt
     6721 tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc gtccgctgtc accctgcaat
     6781 gataggggat ctcaaatatc aactacaagc gttatgctca tctaaccccg aacaaaacga
     6841 agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag gatcagctta
     6901 aaatatctgg ttgttatatt ttttgtaaaa aagaatgtag tcgaaaatga atgcctttag
     6961 atgtcttgat catgatatga tcttaaaaat tgtcttatat agcgagcaca gctaccagaa
     7021 taatctgttt cgtgtcacta tttgtttgtg cgattgcggt ttgggatttt tgtgggtcgc
     7081 agttctcacg ccgcagacaa tttgatgttg caatcgcagt tcctatagat caagtgaact
     7141 taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg ggtgtttgct
     7201 gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt ttcacacttt
     7261 cccctgctta cccacccaaa aaccaatcac caccccaatc actcaaaaaa caaacaaaaa
     7321 taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg taaaccgctt
     7381 ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg acgctacgta
     7441 acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac ggcgatattt
     7501 ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat tttgagtgag
     7561 aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat ttaaaactga
     7621 tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt
     7681 tccgcaaaaa tgggttttat taacttacat acatactaga attgggccgg ccttaattaa
     7741 gtggaattcg atatcaagct tgcacaggtc ctgttcgata acgtcgtact cgggaatcac
     7801 ctttaacttg tccgctattg ggtatttgtc cttaaacaca ttcgtaatct ctcccacaat
     7861 cattgccctg gcctctgcaa tgtaaaaagt atttataaaa tatcgctggc tctaaagaaa
     7921 acatattaat caaagtattg aggctttatc aatctaacat ttacaagtag tcagaccaca
     7981 atttttagac tgcacttctg cgtaagtaaa tgaaatgaaa tggtgaccta ctcttgggta
     8041 gccgtactgt tatatgctga cgagccttca cctgggcctg ctttttctcc tgtttcgtct
     8101 gatgcgtcgg atgcggctgg actggctgat tgttgggatt gggttttgga tgctgctccg
     8161 aaaggtgggc cacagctttt gcgatggtgg tgcccactat gcggttgctg caggaactac
     8221 agctgaatgt tcgctttatt gtctgcagct cctggggctg gacgaaggcc acgccctcac
     8281 ccggtgcggc catctcctgc ggcaaaacgt atgtgggatt cctgtccaag tagctctcga
     8341 tgcccaccat aatgcgctcc agcaggttgg ccaggtgcgg gtctagctgc ggcggattcg
     8401 tcgtctggtt cactgagttt agggtctgca gaaagaagtg tgcctccatg gtaagcggat
     8461 tcatctgcac gatcagcggg ctttagtcta aaaattcgaa tgatacaaac tcgtactcac
     8521 gtaaagcagt ttgtttggca tcatctcggc catcgtctta ttcttcttcg tggcctttgt
     8581 taccggagcg gctatgttgg gcgcggtgga gcccggaagc tgaaaggtta atcgaatgcg
     8641 ttaacttttc tgcaactcga aagtttgccg cctttgttcg actgccaata actgttgatt
     8701 cgaaaattcg aatcgaagcg gttgaatttc gtagggtggc caactacacc aaagttcgcc
     8761 ggcggtgtat gcatgtgagt gtgagtgtga cagtgggtgt acgtgtgctt taagcgacac
     8821 aatcgcaccg cgatgtacat atttttgtag aaatgtgtgt gcatttttcg tccggatcac
     8881 ggctccataa aataaatcat atcacagggc tcagggattt gtgagtagaa gttcccgcca
     8941 cttaaacgtc acttgtgaaa tcgccatcga cggtcaactt ttactcacac tggtcttccg
     9001 cggacggacc gtctcaaagt actgcctttc tgcgttggaa aacatcgcct ttttcgtcca
     9061 aaaggagtcc ccaggttcga tccgcatggc gttgtgcgtg cgtgcctttc ttttcaaatg
     9121 attacggcta ttaacttggg ggcgttaagt tggaaacacg taaattgcag actgcgatta
     9181 gagtgaccat gagtaggagt tcaaaatctc ctgacatcat tttcttaaaa cctgctttgt
     9241 tttttacatt tctatttaat ataactccta tttgaataaa aaaacaaaac aagtttagat
     9301 gttaagatat taactacatc ctttgctcca aagggagagg ggaagttatg gagttaatta
     9361 atttgctgtt ggaaatcaat atggagtcag aaatataatg atttactaaa ccttattgaa
     9421 tcggtaacga tgcgaattta tattaaaata gcttttatga aacattcaac aaaaatatta
     9481 ttaatgttgg cccactttag caaccggtta ggtctaccgg ttgggcaagc aaagattcac
     9541 gccctggttc gagtcccaac tagtcctgca aaataccgca gcaagtttta gagagaccaa
     9601 gtgccattac ctctcccact tcagttatcg gttatgcggc gtttaagtcg acagcttgcc
     9661 gtctctagct ccggtgccta tataaagcag cccgctttcc acatttcata ttcgttttac
     9721 gtttgtcaag cctcatagcc ggcagttcga acgtatacgc tctctgagtc agacctcgaa
     9781 atcgtagctc tacacaattc tgtgaatttt ccttgtcgcg tgtgaaacac ttccaataaa
     9841 aactcaatat ggtgagtact ttaaaaaaaa atctagtgaa ataatgctga aaagaaattt
     9901 gtgtgggcaa aattcaatgg gcaaaaacgc gatgcggctt tttctcaaaa tggcggccgg
     9961 cctgcgtttt ttcctcaaaa gtgatgacgt catgcctgtt tttttttttt gttcgcaatg
    10021 aggaatggct cttaaaatct agataaaaaa aatattcatt atttctatgc tgctggaacg
    10081 cttcattaat cttaaaaatt ctaaattcgg ttaccatgat acttcgacgc ataactgtag
    10141 attttggata gaattaaaga gaaaatggcg agagagtaaa attccggcgt cggcaaagta
    10201 gagcaaaaaa atcagtatac catttagcta cctctctcac tcgcacgcag tgccggctca
    10261 agttgggcgc ggctctgcaa ttatcgattt tcttggggtg tgtaactaat catccgtttt
    10321 cccttcctcc tcatccacag cgtgaaggta cctaatccaa aatgggatca tcatcagacg
    10381 acgaagctac ggccgatgcg cagcatgcag caccaccaaa aaaaaaacga aaagtagaag
    10441 acccacgatt tatgtaccca tacgatgttc ctgactatgc gggtatgaaa aacatcaaaa
    10501 aaaaccaggt aatgaacctg ggtccgaact ctaaactgct gaaagaatac aaatcccagc
    10561 tgatcgaact gaacatcgaa cagttcgaag caggtatcgg tctgatccta ggtgatgctt
    10621 acatccgttc tcgtgatgaa ggtaaaacct actgtatgca gttcgagtgg aaaaacaaag
    10681 catacatgga ccacgtatgt ctgctgtacg atcagtgggt actgtccccg ccgcacaaaa
    10741 aagaacgtgt taaccacctg ggtaacctgg taatcacctg gggcgcccag actttcaaac
    10801 accaagcttt caacaaactg gctaacctgt tcatcgttaa caacaaaaaa accatcccga
    10861 acaacctggt tgaaaactac ctgaccccga tgtctctggc atactggttc atggatgatg
    10921 gtggtaaatg ggattacaac aaaaactcta ccaacaaatc gatcgtactg aacacccagt
    10981 ctttcacttt cgaagaagta gaatacctgg ttaagggtct gcgtaacaaa ttccaactga
    11041 actgttacgt aaaaatcaac aaaaacaaac cgatcatcta catcgattct atgtcttacc
    11101 tgatcttcta caacctgatc aaaccgtacc tgatcccgca gatgatgtac aaactgccga
    11161 acactatctc ctccgaaact ttcctgaaat aagcggccgc ataggccact agtggatctg
    11221 gatccttctt aagaagagct ctaaagcttt atctagagtc gacctcgaac gttaactcga
    11281 ggcctataag atctcttgca tgctacctgc aggataccgg tataacgcgt atagctagca
    11341 ttcgaatcgg aaatcaatcg aattctcgat caatgttttg cgaaaaaaaa tgaaacgaga
    11401 acaaatgaat tcagttgtaa aagatttgtt ctcttaacaa cgtaatcaat aaagtcggat
    11461 ccaatttgtt cacaagtctc ctagcattcg aatcggaaat caatcgaatt ctcgatcaat
    11521 gttttgcgaa aaaaaatgaa acgagaacaa atgaattcag ttgtaaaaga tttgttctct
    11581 taacaacgta atcaataaag tcggatccaa ttttgtcaca agtctcctag gtctgcgatc
    11641 gcaaaggcgc gccatgcagc caagctt
//
