LOCUS       sequence-81517-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-OTUD7B
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..85
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat ctgccaccat
       61 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
      121 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatgttct accatacatc
      181 cagctggctg aaagtacttg aggatcacat gaccctggac atggatgctg ttctgtcaga
      241 ttttgtccgt tccacaggag cagagccagg gctagcgcga gatctcctag aaggaaagaa
      301 ttgggatgtg aatgccgccc tcagtgattt tgaacagcta cgtcaagtcc atgctggaaa
      361 cctaccccca tcctttagtg aggggagtgg tggctccagg acccctgaaa aagggttttc
      421 tgacagagag cctactcgcc ctccccgacc catcctccag cggcaggatg acatcgttca
      481 agaaaaacgc ctgtctaggg gcatctccca cgccagctcc agcattgttt ccctggcccg
      541 gtcccatgtc tcctccaatg gtgggggtgg ggggagcaat gagcaccccc tggaaatgcc
      601 catctgtgcc ttccagcttc cagatctcac tgtatacaat gaagacttcc gcagcttcat
      661 agagagagac ctcattgagc agtccatgct ggttgccttg gaacaggcag ggcgtttgaa
      721 ctggtgggtg agtgtggatc ccacctctca gaggctgctt cctttggcaa ctactggaga
      781 tgggaactgc ctcctgcatg cagcctccct tggaatgtgg ggtttccatg atcgggactt
      841 gatgctgcgg aaagctttgt atgcactgat ggagaaggga gttgagaagg aagcgttgaa
      901 aaggcgctgg aggtggcagc agacacagca gaataaagag tcagggctgg tatacacaga
      961 agatgaatgg cagaaggagt ggaatgaact gatcaagctt gcctcaagtg aaccccgaat
     1021 gcatctaggt accaatggag ccaactg
//
