LOCUS       sequence-81534-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: MSCV-N-Flag-HA-IRES-PURO
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(161..180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     657..677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
       61 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      121 gttttccttt gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac
      181 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
      241 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
      301 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
      361 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
      421 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
      481 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
      541 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
      601 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
      661 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
      721 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tgcatcgatg tcgacctgca
      781 gccaagctta tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
      841 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
      901 aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat
      961 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     1021 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatca
//
