LOCUS       sequence-81552-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant 
            expression of Cas9 and multiple cloning sites for insertion of guide
            RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGC-pcoCas9
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(248..500)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(509..556)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attgaaaatc tcagaattcc tcgagtacgt aggatccatt taaattctag aggcgcgccg
       61 atatcctctc ttaaggtagc gagctcttaa ttaataggga taacagggta attaactata
      121 acggtcctaa ggtagcgatg gcaaacagct attatgggta ttatgggtgg ttctttatgc
      181 ggacactgac ggctttatgc ctgcaggtcg cgagcgatcg cggtaccgcc cgggcgtcga
      241 caggcctgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
      301 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      361 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      421 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      481 tgccaaatgt ttgaacgatc tgcagtcact tcttcttctt agcctgtcca gccttcttgg
      541 tagcagctgg tctcttatct cctccaagct gagaaagatc gattctggtc tcgtaaagtc
      601 cggtgataga ctggtggata agggtagcat caagaacctc cttggtagag gtgtatcttt
      661 ttctatcgat ggtggtatcg aagtacttga aagcagctgg agcaccaagg ttggtaaggg
      721 tgaaaaggtg gatgatgttc tcagcctgct ctctgattgg cttatctctg tgcttgttgt
      781 aagcagaaag aaccttatca aggttagcat cagcaaggat aactctctta gagaactcag
      841 agatttgctc gatgatctca tcaaggtagt gcttgtgctg ctcaacgaaa agctgcttct
      901 gctcgttatc ctctggagat cccttcaact tctcgtagtg agaagcaagg taaaggaagt
      961 taacgtactt agatggaaga gcaagctcgt ttcccttctg aagctctcca gcagaagcaa
     1021 gcattctctt
//
