LOCUS       sequence-8158-ex        9148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Mayr C, Hemann MT, Bartel DP
  TITLE     Disrupting the pairing between let-7 and Hmga2 enhances oncogenic 
            transformation.
  JOURNAL   Science. 2007 Mar 16. 315(5818):1576-9.
  PUBMED    17322030
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Mar 16. 315(5818):1576-9."
COMMENT     Sequence Label: pcDNA3.1 Hmga2 wt
FEATURES             Location/Qualifiers
     source          1..9148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4742..4759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4748..4972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5018..5446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5105..5124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5315..5336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5455..5475)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5460..5789
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5640..5775
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5702..5721
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5856..6650
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5910..5929)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6520..6539
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6824..6945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6861..6880)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6915..6934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6994..7010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6994..7010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7007..7029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7018..7034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7042..7048,7049..7066,7067..7072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7087..7108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7225..7242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7396..7981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7476..7495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8152..8943,8944..9012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8775..8794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9013..9117)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcccg
      961 ggatcgcaac tcccgagcct ttgcggagag agcaaccctc tccgcctcca actcttccct
     1021 ctccttcgct tcccgcctcc tctccctacc tccacctcta cctccgccac ccactgcccg
     1081 cagcgcctcc tcctttcctc ctcctcctct cctcctcctc tcttcctcct cccctctctc
     1141 tttttggcag ccgctggacg tccggtgttg atggtggcag cggcggcagc ctaagcagca
     1201 gcagtagtcc ccggcgcact cgccagctcg cctgctctcg ccgctcttgc cctctccagc
     1261 tctctacatc ccgtctcccg aaaggtgctg ggcggattcg gggcggcgga ggccgagcgg
     1321 ctgcagcggc ggtaccggta gaggcagtgg tagcggcggc gggaggcagg atgagcgcac
     1381 gcggtgaggg cgccgggcag ccgtccacat cagcccaggg acaacctgcc gccccggtgc
     1441 cacagaagcg aggacgcggc cgacccagga agcagcagca agagccaacc tgtgagccct
     1501 ctcctaagag acccagagga agacccaaag gcagcaaaaa caagagcccc tctaaagcag
     1561 cccagaagaa agcagagacc attggagaaa aacggccaag aggcagacct aggaaatggc
     1621 cacaacaagt cgttcagaag aagcctgctc aggagactga agagacatcc tcgcaagagt
     1681 ccgcagagga ggattagggg gcgccgacat tcaatttcta cctcagcatc agttggatct
     1741 tttgaaggga gaagacactg cagtgaccag ttattcttaa ctgccacggt ctttctactt
     1801 cctgcggggt ggggcggggg cggggctggg cgaggggcgg ggccggggtg ggcgaaatcg
     1861 cataaccttg agaaggacta tattaatcac tttgtaatcc cttcacagtc ccaggtttag
     1921 tgaaaaactg ctgtaaacac gggggacaca gtttaacaat gcaactttta atgactgttt
     1981 tctttttcct taacttacta atagtttgtg gatctgataa gcaagggtgt gtggttgaag
     2041 aaaacctctg tggtgggctt aatcagtcac tacatgcaaa ccctaaaccg gcaccctggt
     2101 gaccgggggc attcgtataa gaaaagcatt gtgtgtgact ctgtgtccac tcagatgcca
     2161 cccccaccat gatcatagaa aatctgctta ggacaccaaa gatgagaact agacactact
     2221 ctcctttctt tgtgtataat cttgtagaca cttacttgat ttttttttct ttttttactt
     2281 ttcaattctg aatgagacaa aatgctggtg tatcttttca tacagctagc aaaccagaat
     2341 aggttatgct cgttttttgc tttgttttgt ttttcaaaaa gggaagtaaa cgagaaccgt
     2401 tgactcctcc atttatggac tcatacacag cagcaggagt gataagccca caagctctct
     2461 ttcccgcctc gggaaatcta cacagccaaa agccacttag ccataaatga cacttgtcag
     2521 ccttgaagca tcggagataa ctagctgagt aaaatgatcc tgttttggaa tttaatgaaa
     2581 aggttaacag tacccaatga acccacccaa gtgatgacat gggaggagcg aaaccgaaat
     2641 ctcttttgct atataaagga cactattttt taaaaaaaaa taataaaaac agctcccgct
     2701 ctctgtcctc tctccctccc ttctctccct cgcctctctc tcctctctat attccctgtt
     2761 cttcattgtg taccagtgtc cgtgaaagac cgcagtacca cttacctcag atgaagcctg
     2821 cgtgttacat cctgtaacac ctttcatttt gacataagat ggctagccga ggtgcattat
     2881 cttggttcgg actgccatct ctgcattcac gctgcacttt tagccagaga tgcaataatc
     2941 cccactcctc aatactacct ctgaatgcta cagtgaattt acagccctgc acttgttaca
     3001 cgctgctaga cacaagccct gcaagagaaa ggaaaaaaaa aagcccacca aaaccaaacc
     3061 aaaccttact gggtcggcat ctcagccatc cccagttctc gaccattctt ctctgtactc
     3121 ttactccgtc tcagcaggct atgcatgttt ctatgacttt tttttttttt aattaaaatg
     3181 ttacaaatgc ttgtggcagc tttcctgcta gattgttaca ttaatttgaa acagttttga
     3241 gtcaagttgc tcctaggttc ttaaggagaa tttttttttc agtgacacta ctttgtatca
     3301 cacacacaca tctgtagtgt tcaaatataa gtctccaagt ttgtacctca aatgaattat
     3361 tgaaacaaat ggacttcctg atttgcaagg aactacctcc acacttccaa aggaacgaac
     3421 ttgcagccta tatcactcat tgatttcctt cccccatgtt tgaaggagct caaacctcac
     3481 ctctccctca ttgaaacatt ttttttggta aaagacactt gatagaaaca caattttttt
     3541 acatactttt gcaaaaataa atgaattaaa atcaagccaa ccttcaaaga aacttgaaat
     3601 tttgctacaa ccagctcagc cttttgccta atgcaatgaa aaaggaaaaa aatagatttc
     3661 taagatttgt tgcctagaag aatatgcttg accgatattt tttcatgtat tttacacaat
     3721 gtgatttttg taaaaaaatg tctcaagcag atttgttttg gacgcttctt gtgtagagtt
     3781 tctatgcctt tctctcctat taagtgtgct gactttccag agtgttaccc actgggccag
     3841 gaggtagttt ctcatagtgg cttgtgtcag tataagttaa tactgaagcc aaatgaaaca
     3901 aacaaacaac catgtctctt ccagctgttt tcagggaggt tacttcaaag gccacgtgcc
     3961 gctctgagac tggcagatgg ctcactgttg tgagtcgcca aaggagctat ggagagatta
     4021 aaattcaaca tgactgttaa caatgcatta aataatcaaa taaacagtgg cttataaata
     4081 tcagattctc attccgggtc ttcggatggg ccttacagaa acctcatttt ggccagctca
     4141 taaaaactga agcagcttct cgtgttggcc agactcggca caccgagcaa tttccatctc
     4201 tgatgaagtt attccttatt tcctgtatgt tgtacaatca aaacacacta ctacctctta
     4261 agtcccagta tacctcattt ttcatactga aaaaaaagaa aaaaaagaga aaaaaaaagg
     4321 cttgtgtcca atggaacagt gagaacatca taaaattttt atatatatag tttatttttg
     4381 tgggagataa attttataag actgtttttt actgttgttg gtcacagcta agtaagactg
     4441 gacatctaac ttctctacca tttctgcaag ctaagtatgt ttgcaggaga aacttatcaa
     4501 gacgattaac tgcatccaac tttctccccg ttcctttgtg tgccctctga cttcgttctc
     4561 tgttccttgt gtagagtcgc tgtctgtgat tgtacttgga cggcttgctt gtggcaatac
     4621 cttctctagt ggattatcac tgtctgcaca ataaacataa cagccaaggg cgaattctgc
     4681 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     4741 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4801 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4861 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4921 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4981 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5041 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5101 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5161 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5221 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5281 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5341 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5401 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5461 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5521 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5581 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5641 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5701 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5761 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5821 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5881 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5941 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6001 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     6061 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6121 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6181 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6241 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6301 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6361 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6421 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6481 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6541 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6601 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6661 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6721 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6781 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6841 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6901 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6961 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7801 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7921 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8041 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8101 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8161 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8221 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8281 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8341 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8401 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8461 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8521 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8581 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8641 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8701 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8761 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8821 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8881 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8941 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9001 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9061 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9121 atttccccga aaagtgccac ctgacgtc
//
