LOCUS       sequence-81589-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant 
            expression of Cas9 and multiple cloning sites for insertion of guide
            RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGC-pcoCas9
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctgtccagc cttcttggta gcagctggtc tcttatctcc tccaagctga gaaagatcga
       61 ttctggtctc gtaaagtccg gtgatagact ggtggataag ggtagcatca agaacctcct
      121 tggtagaggt gtatcttttt ctatcgatgg tggtatcgaa gtacttgaaa gcagctggag
      181 caccaaggtt ggtaagggtg aaaaggtgga tgatgttctc agcctgctct ctgattggct
      241 tatctctgtg cttgttgtaa gcagaaagaa ccttatcaag gttagcatca gcaaggataa
      301 ctctcttaga gaactcagag atttgctcga tgatctcatc aaggtagtgc ttgtgctgct
      361 caacgaaaag ctgcttctgc tcgttatcct ctggagatcc cttcaacttc tcgtagtgag
      421 aagcaaggta aaggaagtta acgtacttag atggaagagc aagctcgttt cccttctgaa
      481 gctctccagc agaagcaagc attctctttc ttccgttctc aagctcgaaa agagagtact
      541 ttggcaactt gatgataaga tccttcttaa cctccttgta tcccttagcc tcaaggaaat
      601 cgattgggtt cttctcgaaa gaagaacgct ccatgatggt gattccaaga agctccttaa
      661 cagacttcaa cttcttagac tttcccttct caaccttagc aacaacaaga acagagtaag
      721 caacggttgg agaatcgaat cctccgtact tctttgggtc ccaatccttc tttctagcga
      781 tcaacttatc agagtttctc tttggaagga tagactcctt agagaatcct ccggtctgaa
      841 cctcggtttt cttaacgatg ttaacctgtg gcatagaaag aacctttcta acggtagcga
      901 aatctcttcc cttatcccaa acgatctctc cggtctctcc gttggtctcg ataagtggtc
      961 tctttctgat ctctccgtta gcaagggtga tctcggtctt gaagaagttc atgatgttag
     1021 agtagaagaa gtacttagcg gtagcctttc c
//
