LOCUS       sequence-81590-e       15210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Gal80 under vesicular glutamate transporter promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15210)
  TITLE     dVGlut-Gal80
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dVGlut-Gal80
FEATURES             Location/Qualifiers
     source          1..15210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5807..5831)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          6013..6079
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    7407..7995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7460..7480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7878..7889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          8087..8152
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8282..8302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8574..8695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8611..8630)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8665..8684
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9732..10346,10417..10548,10752..11067,
                     11129..11783,11858..12131,12533..12604))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     13056..13074
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    13299..13368
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(13455..14043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13535..13554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14214..15005,15006..15074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14837..14856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15075..15179)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gcaggctccg aattcgccct
      121 tgtgatcccc accggaattg cggcctcagc agattaatct ggccggccta cttaattaag
      181 tggaattcat aataaatgta ttaaaaccga tttaaggcca cggaaaagca cggactgccc
      241 gttcttgcta aatgatgtgt gacaacattt ttattgcccg ctctcggtca cccttatcct
      301 gcgccgtgga cggggtaaaa gccaacgtgc gccaacatta atcaaggcat tctaaagatt
      361 caggctagca tttatgagca aactcatgca ttttgagggt aaaactcaaa aaacgaacgc
      421 aacaacagaa agcggcacac gccgggcgac aaaagcattt gcttccggta taccgccgcc
      481 tgtcaccagg acttctacac tcgacaaaaa atatcacata gcattgggaa aatacttaca
      541 atgtcttttt tcaaatgaga aattaaatat gtagtttcta tttatggaaa caacatggga
      601 ctttctctgc atactgctgc agtatttttg tttagtgtaa taaatatgag gttttgtgtt
      661 ttagtaggtg ggttgcctgc gcattgtagc ttgaaataaa gttgcactga ctgacgcttt
      721 tcgccttttg tattctgctc ttattgttgt tcggcaagcg aagagaaaaa tgtattccga
      781 atgaataaat gcaatatact ttctatgacc ctaatggatt tcgttgttgt tgtacgacgg
      841 acaaagagtt gagtcctaaa tggaggcata tgctaattag gatgggtaag gcgattcctc
      901 caagtggggt agtagtagga aactatggaa atatatgata gtttcatgtg tctataaatc
      961 caaaaaatac agaagctgta agataaatct cccaccgaac agacatctca aaatcccccg
     1021 tttcatagtt cgcccctcgc agtttccatc atatctcaga cgcttccagc tatttgcaca
     1081 caaacaaaaa taaatattaa atctcagaaa tcgtttttgg gcagcgacac acccttaacc
     1141 cccgacctgc gtgattcccc cagtcccaag gattcgaaat gttactccgc ttactatgtg
     1201 ccacgcttat cgacgagcac agcgtttagt ttgatgattg agtgggtgga catgtggatt
     1261 gacagcatga gtctgctgaa tccacagaag ctcatcaatt tcccgcctcg gtgggaaaac
     1321 cactcgcata cttttgacga tttcaagctg aaattcgaga gcttttaaag tcgtcacagt
     1381 ttcggctggt aattaaaacc gcgtgaaacc ctttcaccga aagtaatcca ccgacgccca
     1441 ttcccgtaat tcactcataa atcagacaac gaaaagccat acatcatacg caccgtgggg
     1501 aatctcccga aaaaagtaca cagagatgac aaagctgcaa aagttatttt gtaattagtg
     1561 caagcggcaa ggacgacggg agaccgccgc gtggataagg acacaacagg cccaacagga
     1621 taatgatgat tacgctggcg caatgaaatt gaagacgact ttctgccgaa gctgcactaa
     1681 ttaccgctga cgtgcgatgg gaactgggag gccagcggaa gtcctcgccc gattccagga
     1741 cgaaactccg tccgggggac tgtcaactca agtttgacac ttttctgctg acaatttatg
     1801 agagcgggcc aacagctaag gttaccgatt tgagcacagt gggtgttaaa gaatcatttt
     1861 gagtgaggaa tattataaat ttgtcgaaat tatagatgaa tatagcttgc aaatctcata
     1921 tgcatataga acttattgta atttaaaaaa tatatatatt tgactagcac ttttgtagat
     1981 tgagcttgta accaatttga atgttcggtt ttttccactg tgcacgatgg gcactgactg
     2041 acactcgatc cggccgacaa cgtcgccact tagtttagag ttttatgagc acaattcgca
     2101 attcgattat tcttcattta cgtcaatcaa tgtgaatggc gcttttagcc gctagccacg
     2161 cacccttaat cgctttgttt aaatagtaaa acgccgcacg gattgggcat ttttcgaaat
     2221 ttaatatcgc agatgctttt attgtgccgc cgtcttgtct gtggaaatct gtctgccccg
     2281 aagtctggct catgccgtag acgtagtatc tgtatctgca aatgtatctg tatccctttc
     2341 gaatgcgaaa atcccgctgc cgttctgttg actttccttt catttcccag ctcggctgtt
     2401 tcctcgtccc gcttcttgtt tatttcgctt tgctgtttac ttccgcaact ttattatagc
     2461 ttttggctga gttgcaactg tttgttggct gtttgcttta tgcttatgag ggttaggcca
     2521 acgcgtcgta ttcgcaacgt ggccaggaca tctggagggg atcctggaat gggacgccga
     2581 acggaagcgg ggatttctat ggggcatgga ttttttctgt cccgtgaatc gatgtggttg
     2641 atgagaatcg aggcagttgc tcacactgtt gaccaggatt attagctact taaagtggcc
     2701 attgagaata ttcaagttga gttgagccta ataagtatat gtgttgtttg gaactggaga
     2761 ttcttttctc tttccttgaa tataataacg aataataata aacgaatcat tctgcttctt
     2821 ggggctaata aaagacaact agctttggtt ttaaataaga tcaatttagg atcgaaaaat
     2881 ccattagagt attctaataa aatgaatcaa atgttttttt accgttagac cgcccagtaa
     2941 ttaatgataa tccattagat agatttcaag ctcccaaacg tgagaattta aaaagcatca
     3001 cgtccgattc gaatcgatca aagccaagag caaaggactt atttatttgt agccggtcag
     3061 acttcacgat tcatattgaa acgctcatcc ataaatcgta gcattttcca aaaattcacc
     3121 aagtttggag ctattttgag cccccatgca gacaggggaa aatattcaaa atggctgaca
     3181 ttctataaaa aggcggcaga aggacgcagg acgtctgtgt gccaggagga aaacaatgga
     3241 aaatggcctg gcaacatgtt aatagcattt cacgcacgac tgagattcgc atgccaaccc
     3301 agagaaagac attcccatgt accaggacct ctgattggtt ctttttggcc aggtggcgtg
     3361 ccattccccc acagctgtcc ccagtctcaa ctgctacagt tgccattcgc cgtcaaccaa
     3421 tgccgaagga cgaaggactc tcgaaggatt ctcgagcctg cgcagctgtc gcctgcgtcg
     3481 cgagcttttc accacagcgg gtgcgaacgg gacggatgcg ttgattatgc tgcggaggag
     3541 cataatcctc tggcaggaca tccatctggg cagacaagaa aggcgaatgc gggcgctttt
     3601 agtttcactt cgtgcgttgt cctgctgagg ccaaaaagcg agcctggagc tgcgggtata
     3661 tagctgggaa aatacatcta aaatatacca ataaatcaga aaatatagtt caaaatatcc
     3721 gaataatcgt gctaatatag caagtgtttc gaaaaagtgc tgccctccgg ctagaagttg
     3781 agaaatctac gtttttctgg caacgcctcg taataccctt gaaaagaaaa tctcgcacat
     3841 tccagtggtt gaaaatttcc agtgtatata ctacgctcct cccacccctt taacacccca
     3901 tcccacattc gcttcatcac tagtccctcc gtacatatac ccctgtatgt gatacattcc
     3961 aggccgcgat ggcagcttat gtatccatct tccgctgcca cctgtcctcc gccgatcttt
     4021 ggctggaggt ccacaacgcg gctgattttg agttggacgt cacacagtct tcagacagag
     4081 ttggattcgg atcaccatcg tcctcgtcgt cgtcgtcggt tccatagaca acctgccgcc
     4141 ggtctcgtgg tcaaactctg tctggatgtt gtcgtcccac atactgagtt cttaattggt
     4201 ttatccaaag ttttaagttt ttcactttag tttcatttct atttttgcgg gcgagtgttg
     4261 cttagaaaat gtgagacttt atataaataa atacaaaaca tctataacga tatattttaa
     4321 cacattacca agaagatata tattgtttta aaaaattctt ctaattatat taaatcacct
     4381 aattccatat ccctttttat cccatttgca gcgtatcgcg tagtgaataa tcaagctata
     4441 taatctgtta tctgtgtacc ttatttgtga tatcctaacc aacaacaaca aaataaatta
     4501 aacgattttc gttgtctact aaaacataat ttaagccaag ttaatcacca aaaatatctg
     4561 tgcttaagtt acgactagag gccgattata taatcttgca ccaactacga aatggattac
     4621 gtaactctgg tgtggcatgt aagtaggcgt tgtggccaaa ggtcaaaagt acaactgttt
     4681 tgggggagga gcttccaatg gaatcatcta aagaattttg tgaaaaacgc caaagaatgc
     4741 gtacatatat actttacatt tagtttcgaa attcctgatc taacaagaaa taagctagta
     4801 taaaaaatgc acaaacttaa aacctatgat tatttccatt gaatttgttt tcttattata
     4861 aattgtaatt ctaataattc caataatcgt aacattgcta actctttcga aagagattac
     4921 ccaaatcaaa agaggtgtca aatttcatgc tcacaccaaa tgaagcataa aacgctatca
     4981 aaaccgctca caatgccaga aatatttaca aaatgctctg ccggctcagt agttacccct
     5041 catcacaatc atttcgaaat tctcgtgaat ttatgaacat aagcggctta caagaaatgt
     5101 gcaaaatgat gaaattgtac gagacttttg cttatcagca gcagaacaca aattgccgga
     5161 cccattaata gccgcataca aatctctttt gcaggcactg taaatgaaaa tgcacaaact
     5221 ccacgagaat cgaaatcata tggcgaccaa gtgaaaatcc cccgctgaga gaacgagcca
     5281 acccgaaaat agagtcatca aatttgtgct ggcttcacgg aaattaaccg ccgccgattc
     5341 gctacgctta tatacacggc aatccgccgt gaagtaggca acaaaatata tttatgcgcc
     5401 caagtgataa cggcgcaaag gcaacgaagc acaacagacg ctgacttggg cgaaaggaca
     5461 gcagcaggag caggactcgt tgatccggag gcaggggcgg agtgaggaca ggcgtctggg
     5521 aaaatcagga ctactagctg agcagcaagg gtacccgccc gggatcagat ccgcggccgc
     5581 ataggccact agtggatctg gatccttctt aagaagagct ctaaagcttt atctagagtc
     5641 gacctcgaac gttaactcga ggcctataag atctcttgca tactacctgc aggataccgg
     5701 tataacgcgt atagctagct ttcctaggtc tgcgatcgca aaggcgcgcc atagtttaaa
     5761 caagccggcg tcccggaaaa cgattccgaa gccaagggcg aattcgaccc agctttcttg
     5821 tacaaagtgg tgataaacgg ccggccgagc tcgcccgggg atcgagcgca gcggtataaa
     5881 agggcgcggg gtggctgaga gcatcagttg tgaatgaatg ttcgagccga gcagacgtgc
     5941 cgctgccttc gttaatatcc tttgaataag ccaactttga atcacaagac gcataccaaa
     6001 cggtaccaaa aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa
     6061 ttcttatcct ttacttcagg ctagcaatca aaatggatta caacaaaagg agtagtgtga
     6121 gtacggtgcc gaatgctgct cccattcgcg tgggcttcgt gggattgaac gcggctaagg
     6181 gttgggccat taaaacgcat tatccagcca tactgcagct gagctcccag ttccaaataa
     6241 cagcgcttta ttcccccaag atcgagacgt ccattgcgac aattcagagg ctgaagttgt
     6301 ccaacgccac agctttccca acactcgaga gcttcgcctc gagctccacg atcgatatga
     6361 ttgtgatcgc aatccaggtg gcttcccact acgaggtggt aatgcccctg ctcgagtttt
     6421 cgaagaacaa tcctaatctg aagtatttgt ttgtggaatg ggcactggcc tgctcgctcg
     6481 atcaggcgga gtcgatctat aaggccgctg cagagcgggg cgtgcaaaca ataatcagtc
     6541 tgcagggacg aaagagcccg tacattttgc gggcaaaaga gcttatctcc cagggctaca
     6601 tcggcgatat aaatagcatc gagattgcag gtaacggagg ttggtacggt tacgagcggc
     6661 cggtgaaaag cccgaaatac atttacgaga tcggaaacgg agttgatctg gtgaccacca
     6721 cgttcggtca cacgatagat atattgcagt acatgaccag ttcgtacttc agccgtatca
     6781 atgccatggt gtttaacaac attccagaac aggagctcat tgatgagagg ggcaatcgcc
     6841 tgggccagcg ggtcccaaag actgtcccag atcatctctt gttccagggc acgctgctca
     6901 acggaaatgt tcctgtgtcc tgttccttta agggaggcaa gcctacgaaa aagttcacca
     6961 agaatctggt catagatatc catggtacaa agggtgatct gaagctggag ggtgacgctg
     7021 gatttgcgga aatctcgaat ctcgttctgt attattcggg cacccgcgcc aatgattttc
     7081 ccctggccaa tggtcagcag gcacccttgg accccggcta cgacgcaggc aaggagatta
     7141 tggaggtgta tcacctccga aactacaacg cgatcgttgg taatatacac cggctctacc
     7201 agagtatcag cgatttccac ttcaacacca agaaaatccc cgagcttcca tcgcagttcg
     7261 taatgcaagg cttcgatttc gaaggctttc ccaccctgat ggatgccttg atcttgcatc
     7321 gcctgattga aagtgtctac aagtcgaata tgatgggcag taccctgaat gtttccaaca
     7381 tctcccacta ctcgctttaa aagcttaatc aacctctgga ttacaaaatt tgtgaaagat
     7441 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7501 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7561 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7621 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7681 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7741 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7801 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7861 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7921 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7981 gggccgcctc cccgcctgaa gcttcctagg gatctttgtg aaggaacctt acttctgtgg
     8041 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     8101 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     8161 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     8221 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     8281 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     8341 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     8401 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt
     8461 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     8521 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     8581 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8641 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8701 tctaccggta ctagttctag agcggccgct ggccacgggt gcgcatgatc gtgctcctgt
     8761 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     8821 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     8881 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     8941 ccattatgtt ccggaggcgc gccctagttc cagtgaaatc caagcatttt ctaaattaaa
     9001 tgtattctta ttattatagt tgttattttt gatatatata aacaacacta ttatgcccac
     9061 catttttttg agatgcatct acacaaggaa caaacactgg atgtcacttt cagttcaaat
     9121 tgtaacgcta atcactccga acaggtcaca aaaaattacc ttaaaaagtc ataatattaa
     9181 attagaataa atatagctgt gagggaaata tatacaaata tattggagca aataaattgt
     9241 acatacaaat atttattact aatttctatt gagacgaaat gaaccactcg gaaccatttg
     9301 agcgaaccga atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga
     9361 atgtgttgcg gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg
     9421 gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg
     9481 gtgttttatg tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt
     9541 ggttagaaga caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt
     9601 ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg
     9661 caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg
     9721 atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc
     9781 cggaagctca cgatgagaat ggccagaccc acgtagtcca gcggcagatc ggcggcggag
     9841 aagttaagcg tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg
     9901 cagctaattt cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg
     9961 taacggaacc atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc
    10021 aagaagaagc cgccaaagag caggaatggt atgataaccg gcggacccac agacagcgcc
    10081 atcgaggtcg aggagctggc gcaggatatt agatatccga aggacgttga cacattggcc
    10141 accagagtga ccagcgccag gcagttgaag aagtgcagca ctccggcccg cagtccgatc
    10201 atcggatagg caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg caattcggca
    10261 atcgttttgc ccagaaagta tgtgtcacag cgataaagtc gacttcgggc ctccctcata
    10321 aaaactggca gctctgaggt gaacacctaa atcgaatcga ttcattagaa agttagtaaa
    10381 ttattgaaat gcaaatgtat tctaaacatg acttacattt atcgtggcaa agacgttttg
    10441 aaaggtcatg ttggtcagga agaggaagat ggctccgttg atattcatca cacccacttg
    10501 cgtgagttgt tggcccaaaa agatgaggcc aatcaagatg gcaaccatct gcaaattaaa
    10561 atgttactcg catctcatta atattcgcga gttaaatgaa atttatttat cttctgcaaa
    10621 actataaact atacatctca ttgaaaaaaa ctaagaaggg tgtggaatca ggcaattcta
    10681 tctaaaatct agcgaatttg tttccaagaa ttgtaagcgt tatatcattt gtttccactg
    10741 gaaccactca ccgttgtctg aataagtcgc acttttacga ggagtggttc cttgagcacc
    10801 gacagccagg atcgccacag gaccgcccgg aactgcatga accaggtggc cttgtaggtg
    10861 tacccattct ccggctgctc cagtggcttc tccagatttt tggtggccaa caactgctcc
    10921 atatcccggg ctactttgct aatggcaaaa ttgtcgcata tcttggcgat ccgatcacgg
    10981 gactcgatct cccgtccggg cacaacggcc aacacctgta cgtaaaagtc cgccggattg
    11041 tagttggtag gacactgggc acccacgctg gataggagtt gagatgttat gtaatactag
    11101 atacccttaa taaacacatc gaactcacta ggaaaagaag tcgacggctt cgctgggagt
    11161 gcccaagaaa gctaccctgc cctcggccat cagaaggatc ttgtcaaaga gctcaaacag
    11221 ctcggaagac ggctgatgaa tggtcaggat gacggtcttg cccttctgcg acagcttctt
    11281 cagcacctgg acgacgctgt gggcggtaaa ggagtccagt ccggaggtgg gctcatcgca
    11341 gatcagaagc ggcggatcgg ttagagcctc ggaggcgaat gccagacgct tcctttctcc
    11401 gccggacaga cctttcaccc tgccgggcac accgatgatc gtgtgctgac atttgctgag
    11461 cgaaagctcc tggatcacct gatccacgcg ggccactcgc tgccgatagg tcagatgtcg
    11521 tggcatccgc accatggctt ggaaaatcag gtgttccctg gccgttaggg agccgataaa
    11581 gaggtcatcc tgctggacat aggcgcacct ggcctgcatc tccttggcgt ccacaggttg
    11641 gccattgagc agtcgcatcc cggatggcga tacttggatg ccctgcggcg atcgaaaggc
    11701 aagggcattc agcagggtcg tctttccggc accggaactg cccatcacgg ccaaaagttc
    11761 gcccggatag gccacgccgc aaactgagtt tcaaattggt aattggaccc tttattaaga
    11821 tttcacacag atcagccgac tgcgaataga aactcaccgt tcttgagcaa atgtttcctg
    11881 ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg tccggttgac cagctgccgc
    11941 catccggagc ccggctgatt gaccgcccca aagatgtcca tattgtgcca ggcataggtg
    12001 aggttctcgg ctagttggcc gctccctgaa ccggagtcct ccggcggact gggtggccgg
    12061 agcgtgccgt agtttttggc ctgcccgaag ccctggttaa tgcagctctg cgaagccgct
    12121 ccgctgtcac cctgcaatga taggggatct caaatatcaa ctactagcgt tatgctcatc
    12181 taaccccgaa caaaaagtac cccgaagtat cctacgaagt aggtttatac ttttatttat
    12241 tttttgtgca tctaggatca gcttaaaata tctggttgtt atattttttg taaaaaagaa
    12301 tatagtcgaa aatgaatgcc tttagatgtc ttgatcatga tatgatctca aaaattgtct
    12361 tatatagcga gaacagctac cagaataatc tgtttcgtgt cactatttgt ttgtgcaatt
    12421 gcggtttggg atttttgtgg gtcgcagttc tcacgccgca tacaatttga tgttgcaatc
    12481 gcagttccta tagatcaagt gaacttaaga tgtatgcaca tgtactactc acattgttca
    12541 gatgctcggc agatgggtgt ttgctgcctc cgcgaattaa tagctcctga tcctcttggc
    12601 ccattgccgg gatttttcac actttcccct gcttacccac ccaaaaccaa tcaccacccc
    12661 aatcactcaa aaaacaaaca aaaataagaa gcgagaggag ttttggcaca gcactttgtg
    12721 tttaattgat ggcgtaaacc gcttggagct tcgtcacgaa accgctgaca aaatgcaact
    12781 gaaggcggac attgacgcta cgtaacgcta caaacggtgg cgaaagagat agcggacgca
    12841 gcggcgaaag agacggcgat atttctgtgg acagagaagg aggcaaacag cgctgacttt
    12901 gagtggaatg tcattttgag tgagaggtaa tcgaaagaac ctggtacatc aaataccctt
    12961 ggatcgaagt aaatttaaaa ctgatcagat aagttcaatg atatccagtg cagtaaaaaa
    13021 aaaaaatgtt ttttttatct actttccgca aaaatgggtt ttattaactt acatacatac
    13081 taggcgcgcc catatgttcg gcttgtcgac atgcccgccg tgaccgtcga gaacccgctg
    13141 acgctgcccc gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc
    13201 accgcgccca gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc
    13261 aactaccgcc acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg
    13321 cccggggagc ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac
    13381 atcgtcgacg gtaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    13441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    13501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    13561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    13621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    13681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    13741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    13801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    13861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    13921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    13981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    14041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    14101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    14161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    14221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    14281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    14341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    14401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    14461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    14521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    14581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    14641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    14701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    14761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    14821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    14881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    14941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    15001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    15061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    15121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    15181 acatttcccc gaaaagtgcc acctgacgtc
//
