LOCUS       sequence-81596-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant 
            expression of Cas9 and multiple cloning sites for insertion of guide
            RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGC-pcoCas9
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(34..332,333..355,356..543)
                     /label=35SPPDK promoter
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
     CDS             555..602
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             609..629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
       61 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
      121 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
      181 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acaaggagca tcgtggaaaa
      241 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
      301 aagggatgac gcacaatccc actatccttc gccccaagct tgggcccaag cttgggtcgc
      361 gccccacgga tggtataaga ataaaggcat tccgcgtgca ggattcaccc gttcgcctct
      421 caccttttcg ctgtactctc tcgccacaca caccccctct ccagctccgt tggagctccg
      481 gacagcagca ggcgcggggc ggtcacgtag taagcagctc tcggctccct ctccccttgc
      541 tccgtggatc catggattac aaggatgatg atgataagga ttacaaggat gatgatgata
      601 agatggctcc aaagaagaag agaaaggttg gaatccacgg agttccagct gctgataaga
      661 agtactctat cggacttgac atcggaacca actctgttgg atgggctgtt atcaccgatg
      721 agtacaaggt tccatctaag aagttcaagg ttcttggaaa caccgataga cactctatca
      781 agaagaacct tatcggtgct cttcttttcg attctggaga gaccgctgag gctaccagat
      841 tgaagagaac cg
//
