LOCUS       sequence-81602-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of E. coli FIS for protein purification (Intein-chiting 
            binding domain fussion, New England Biolabs pTXB1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-FIS
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..182)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 gcagccggat ccccttcctg cagtcattga agctgccaca aggcaggaac gttggatggt
       61 tcccatcctg ccaaggaggt gtggggctgc aaacatttat acgtcttgcc gttatatgtg
      121 accaattgtc ccgcagtata agctgtgttg acctgccaag cggatacacc aggatttgtc
      181 gtgaggcctg agttcagacc ggtgaggcca gtagcgtggc tgacgaaccc gttcgtgata
      241 aacgcgtggt ctgccgtgtc gacacgaagg ctatacaccg gctgcacgcc ggcgtcggtg
      301 acactggcga ctttcgcgta gtagaaccgc ccgtcggtca gctcgtcggc gatagcttgg
      361 gcgtccgggt ctcggtggtg tgcttccaag aaacgcacca gtccagggac gccgactgtg
      421 taggttgtgg gcgcaaattc gggtttcccg cgggcaaaac ctgcacagtc gacgctgaat
      481 gcgctgcgtt gaatcaccgc gtaatcgccc ggcttgattt cgtcgatcag cttccacagc
      541 agggtcggca ccccggcgac gtcgaccaaa cacaacaacg ggtggttcgc ggtgcccgtc
      601 acacgcagac cttcgaccgt acgcaccgtg tacaccggat gctcgccgga gtggaacagc
      661 cggtcggcga gcacgggatt gccatgccgg tcaaggactt tcaggtcgat ggcgttgtca
      721 ctgttgggcc gcgcacccgg cacgatgtcg gcgatgcgta ccgactcgcc ctcgggtagg
      781 gcaactagtg catctcc
//
