LOCUS       sequence-81631-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  First 375 amino acids of the peptidase, which contains the soluble 
            periplasmic peptidase domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Brotherton CA, Balskus EP
  TITLE     A prodrug resistance mechanism is involved in colibactin 
            biosynthesis and cytotoxicity.
  JOURNAL   J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. 
            Epub 2013 Feb 20.
  PUBMED    23406518
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja312154m";
            journalName: "J Am Chem Soc"; date: "2013-03-6- 6"; volume: "135"; 
            issue: "9"; pages: "3359-62"
COMMENT     Sequence Label: pET29b-ClbPpep-CHis
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagat atttgccaat gcgcagataa
       61 ttatcgatat ccgcgcaaag ctgtagtatc agattcgaat tcatatttgc cagcgcaaca
      121 atgccaatct gctgatccgg tcgcaacgca atgcaagaag aaaagtttgg attctgccca
      181 ccgtgactga tgtaagggcc ttgattctgg tcgataaacc aaccgctggc atagaggata
      241 cgattgtctg cggcaagcgg aacatcacta ttaccacgcc aactgttact catcgcctca
      301 cgcagcgttg ccggcaaagc ctttctgtgc aaccaggcgt ctatccatat ttccatatca
      361 ggcagagtgc tatggatata ggcggcagga acatggttcc gggccagagg cgcatgaaac
      421 agaacgggtt tgccgaatcc cagtttatag ccgcttgcct tgttgacaat aatctcatcc
      481 cccttaactg ccacagtcgc cgacatgccc agcggctgcg tgagtcgttc cgcaatgacc
      541 tctgtaaagg tttttcccgt cacattttca atcaccgcgc ccaacacatc ataattggcg
      601 gaggcatagc taaacttcgc acccggcgca aacagcagat tctcgttgcg cagttgctgt
      661 gccacagcgc tcccaggcat agggttttcc agccgagcca gtgttgaaaa aggcaatcct
      721 gatgtatgat aaaggaaatc agccacggtc agggaagcag gttttccctg ataattcaag
      781 cgcatttccg gcagataggt aatgatatca tccccttgcc ggagtctgcc ttcctgaatc
      841 agtatttgca ccacaagtcc ggtaaacgcc ttactcatcg atcccagctc gtaaactgtg
      901 tctagagtat tcgctttctg actagccaca tcggcaacac cgtagacaaa tcgctgacgt
      961 accccc
//
