LOCUS       sequence-81632-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untagged, full-length protein for in vivo peptidase assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Brotherton CA, Balskus EP
  TITLE     A prodrug resistance mechanism is involved in colibactin 
            biosynthesis and cytotoxicity.
  JOURNAL   J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. 
            Epub 2013 Feb 20.
  PUBMED    23406518
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja312154m";
            journalName: "J Am Chem Soc"; date: "2013-03-6- 6"; volume: "135"; 
            issue: "9"; pages: "3359-62"
COMMENT     Sequence Label: pET29b-ClbP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt actcatcgtc ccactccttg
       61 ttgtgtaata gaattcgttt aatttgatga tttaatgtca gaacgaaagc taacaggata
      121 attccgaacg gtatcgccaa cacgctcgat ggaccccata ccaagataaa acgccagtca
      181 agtcctggag atagtatacc cggtgcgaca tagagcatgg cggccacgag cccaggaacc
      241 gccaaggcga tgatatagtc acgtacgcgt aaacgtaacc tctgctgttt tccagggcca
      301 tgcgccgttg cgcgataaac acggaaagca ccgcgcacta caaccaccgc cccccaaaaa
      361 cacagcaaca acgtgaggta gacgaaaagg gtatcggtgg ctgtaattgc atcaccagcg
      421 ccgtcagcat atttgccaat gcgcagataa ttatcgatat ccgcgcaaag ctgtagtatc
      481 agattcgaat tcatatttgc cagcgcaaca atgccaatct gctgatccgg tcgcaacgca
      541 atgcaagaag aaaagtttgg attctgccca ccgtgactga tgtaagggcc ttgattctgg
      601 tcgataaacc aaccgctggc atagaggata cgattgtctg cggcaagcgg aacatcacta
      661 ttaccacgcc aactgttact catcgcctca cgcagcgttg ccggcaaagc ctttctgtgc
      721 aaccaggcgt ctatccatat ttccatatca ggcagagtgc tatggatata ggcggcagga
      781 acatggttcc gggccagagg cgcatgaaac agaacgggtt tgccgaatcc cagtttatag
      841 ccgcttgcct tgttgacaat aatctcatcc cccttaactg cc
//
