LOCUS       sequence-81647-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Joseph J, Tan SH, Karpova TS, McNally JG, Dasso M
  TITLE     SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
  JOURNAL   J Cell Biol. 2002 Feb 18. 156(4):595-602.
  PUBMED    11854305
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Feb 18. 156(4):595-602."
COMMENT     Sequence Label: pEGFP-C2 RanGAP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..159)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(169..210)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccaaactc aatggtgatg gtgatgatga ccggtacgcg tagaatcgag
      181 accgaggaga gggttaggga taggcttacc ttcgaaccgc gggccctcta gactcgagcg
      241 gccgccactg tgctggatat ctgcagaatt gcccttgacc ttgtacagcg tctgcagcag
      301 actgtggcgg gcgaaggagc aggattccag ggcgctgttg ggcttggtca cgaacgccag
      361 cagcaggggt gcaagggcct tggggaaata gtcctgctgc accatgtggt tcagcgccat
      421 cagggggccg tacaggttgg caatggcctt gaccttgtct tcactcttga gcagacccat
      481 gtgcacgagg agcctggtga ggaaggtgtt ggagttgaag gacgaggagt tgaaagcctt
      541 ctgcatcagg gcatctactg catcctgcac tgccatcctc acagtagctt cgtccttgaa
      601 cacagatgac acctttagga aggcagagac caccttctcg gggtcagacg tgtcagtctg
      661 ctgggctatc agcacggagc tcttgggccc taggcgcagc agcttctctg gagagggaaa
      721 agccaggaag gtggagacgt ctgcaggagg tggggaggac agcacgggag ctggctcccc
      781 agtgttaggg tccagaatct tccgtgaggg cgtggctgac ttctctccct gccctcgctg
      841 ctgaggctct tcttcctcct cctcctcctc ttcttcctcc tcttcc
//
