LOCUS       sequence-81648-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His tagged Drosophila Puc fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: His Puc (aa 1-393)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        109..127
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    128..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             208..225
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
       61 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta atacgactca
      121 ctatagggga attgtgagcg gataacaatt cccctgtaga aataattttg tttaacttta
      181 ataaggagat ataccatggg cagcagccat caccatcatc accacagcca ggatccgatg
      241 tgtgtgaatc gagaaacgca gagcagcagc aacaataata gcaacaacac tacaacggca
      301 acgtcaacag cacgcaacaa caacagccac aacggacgct gtgagtgcga cggagacggc
      361 gacagcgtga gtcatataca aagtgaaatg aaaatgcgga taaagcgaga agcgccgtgt
      421 cttctgttat ttatgccaat ccaaaatacc aacaacaaca atcgcattgg tgccaatcag
      481 aaggactatc ccaacaagcg gtccagggag aatctggcat gtgacgaagt gacatcgacg
      541 acgtcatcat ccacggcaat gaacggcggc ggacggacgc cggcgctaac ccgcagctgc
      601 tccagtccgg cggtctacga tatagaaaca caccccgcct cgcccgtttt cccgcatcta
      661 ttgctgggca atggacgcga cgccgacgat cccagcagcg tgggcgccaa ctgtgtgctg
      721 aacgttacct gccaaagtcc caatgagagc catctgcagg gcctcaagta catgcaaata
      781 cctgccagcg atacgccaca tcagaacatc aagcagtact tccaggaggc ctacgacttt
      841 atcgaagatg cacggaaaac ggg
//
