LOCUS       sequence-81682-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His tagged Drosophila Puc fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: His Puc (aa 1-393)
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    159..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc tgccatatgt
      121 atatctcctt cttatactta actaatatac taagatgggg aattgttatc cgctcacaat
      181 tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
      241 tgttcgactt aagcattatg cggccgcaag cttgtcgact cagatgccac tgctgctgct
      301 tgtggagctg ggtgaatcta gttctgaaac cgacgacgct gccgccgaag tgctgggcgc
      361 ctcgtcatcc ggggtaattg gcggcgaggt caatctggat gagcagttac tacccgccag
      421 gctgccggag ccgctgtcca catcatcgta atcaaaccca tcctcgtcca tagcctcgct
      481 atccgacttg gatttacccc gttcccgctg ttcccgcttc tcctctatct cctcctcctc
      541 tggctgctcc accagttgac tagattgggt gcttaatccc acagaagatg acgaaggatt
      601 gctaggacta ttcaggtgcg gggttgcggg cgccaggact ccgctcttgc gcaaattctg
      661 ctccagctcc agcagctggc ccatgaagtt cagattgggc gagatgatcg gccgggccac
      721 tttcaccagc ttgtaggcct ccagcaggga gagcgacttg taccgcatga cgtaggcgat
      781 ggcaatggtg gcgctccgcg aaatccccgc gtggcagtgc agcagcactc gggaacccgt
      841 tttccgtgca tcttcgataa agtcgtaggc ctcctggaag tactgcttga tgttctgatg
      901 tggcgtatcg ctggcaggta tttgcatgta cttgaggccc tgcagatggc tctcattggg
      961 actttggcag gta
//
