LOCUS       sequence-81814-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Puromycin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-puro
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..695)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(179..199)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     676..695
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(705..1021)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      719..854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ccctttttct ggagactaaa taaaatcttt tattttatcg atgcatgggg tcgtgcgctc
       61 ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg
      121 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
      181 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
      241 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
      301 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
      361 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
      421 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
      481 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
      541 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
      601 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
      661 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtaa gctttttgca aaagcctagg
      721 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
      781 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
      841 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
      901 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
      961 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     1021 cctaactg
//
