LOCUS       sequence-8184-ex        4245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ13
FEATURES             Location/Qualifiers
     source          1..4245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(163..191)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     939..959
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1685..1707)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1807..1826
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1904..1922)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1990..2094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2095..2163,2164..2955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2313..2332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3126..3714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3615..3634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3868..3885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4002..4023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4038..4043,4044..4061,4062..4068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4076..4092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4081..4103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4100..4116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4100..4116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgagggta
      121 ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      181 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      301 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca
      361 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa
      421 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      481 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca
      541 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      601 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      661 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      721 gttgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc
      781 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      841 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      901 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      961 gctgctggga ttacacatgg catggatgaa ctatacaaat agcattcgta gaattacgcg
     1021 tcctaggcct gaattcctag gaatttattt tgataagtct cgcgtatttt atttgtaata
     1081 atnttataat aagatcaatt tacatttata ctgataaaat tgacacaatt acacttttta
     1141 ttataaaata ttttaaaata tttctatcaa aaggtttatt tttgttttaa atataatggt
     1201 tttttttgta aaaaatactt atttcgtttg ataatcttta ttcagagatt tgaataattt
     1261 taaataaagt agtttagttt tcatataaaa gttaatggta tatataaata tttaaatagt
     1321 aaagattgaa tcagtattca aaatgacacc taaaagttat tctatttcta gctataacaa
     1381 taataataaa gataattatg atacagattt agaaaaaaaa ataaggttaa attaattaat
     1441 tctattttta attaaaatga tttaagggag ttaaatgaaa cagaaacaga ttgggtctct
     1501 atatatttag tttatgtaat gttatttttt ggtggaatac aaataacggt atattttact
     1561 tcaatgtggc catacttatc aacaataaat agtaaggcaa atgtagatac tttagggtgg
     1621 gttgtagcac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     1681 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1741 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     1801 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     1861 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta
     1921 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2101 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2161 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2221 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2281 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2341 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2401 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2461 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2521 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2581 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2641 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2701 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2761 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2821 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2881 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3481 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     3541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3841 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3901 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3961 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4021 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4081 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt
     4141 taaacatgat cttactaact aactattctc atttaaattt tcagagctta aaaatggctg
     4201 aaatcactca caacgatgga tacgctaaca acttggaaat gaaat
//
