LOCUS       sequence-81872-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Is a biosensor for PI(4,5)P2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Varnai P, Balla T
  TITLE     Visualization of phosphoinositides that bind pleckstrin homology 
            domains: calcium- and agonist-induced dynamic changes and 
            relationship to myo-[3H]inositol-labeled phosphoinositide pools.
  JOURNAL   J Cell Biol. 1998 Oct 19;143(2):501-10.
  PUBMED    9786958
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1998-10-19"; volume: "143(2)"; pages: "501-10"
COMMENT     Sequence Label: PH-PLCD1-GFP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      588..597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(639..660)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(900..919)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 acggcatgga ctcgggccgg gacttcctga ccctgcacgg cctacaggat gatgaggatc
      121 tacaggcgct gctgaagggc agccagctcc tgaaggtgaa gtccagctca tggaggagag
      181 agcgcttcta caagttgcag gaggactgca agaccatctg gcaggagtcc cgcaaggtca
      241 tgcggacccc ggagtcccag ctgttctcca tcgaggacat tcaggaggtg cgaatggggc
      301 accgcacgga gggtctggag aagttcgccc gtgatgtgcc cgaggaccgc tgcttctcca
      361 ttgtcttcaa ggaccagcgc aatacactag acctcatcgc cccatcgcca gctgatgccc
      421 agcactgggt gctggggctg cacaagatca tccaccactc aggctccatg gaccagcgtc
      481 agaagctaca gcactggatt cactcctgct tgcgaaaagc tgacaaaaac aaggacaaca
      541 agatgagctt caaggagctg cagaacttcc tgaaggatcc accggtcgcc accatggtga
      601 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      661 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      721 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      781 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      841 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      901 acgacggcaa ctacaagac
//
