LOCUS       sequence-81877-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-PIP3 binding control for PH-Btk-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Varnai P, Balla T
  TITLE     Visualization of phosphoinositides that bind pleckstrin homology 
            domains: calcium- and agonist-induced dynamic changes and 
            relationship to myo-[3H]inositol-labeled phosphoinositide pools.
  JOURNAL   J Cell Biol. 1998 Oct 19;143(2):501-10.
  PUBMED    9786958
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1998-10-19"; volume: "143(2)"; pages: "501-10"
COMMENT     Sequence Label: PH-Btk(R28C)-GFP
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      615..624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(666..687)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccaga
       61 aagaagaagc tatggccgca gtgattctgg agagcatctt tctgaagcga tcccaacaga
      121 aaaagaaaac atcacctcta aacttcaaga agtgcctgtt tctcttgacc gtgcacaaac
      181 tctcctacta tgagtatgac tttgaacgtg ggagaagagg cagtaagaag ggttcaatag
      241 atgttgagaa gatcacttgt gttgaaacag tggttcctga aaaaaatcct cctccagaaa
      301 gacagattcc gagaagaggt gaagagtcca gtgaaatgga gcaaatttca atcattgaaa
      361 ggttccctta tcccttccag gttgtatatg atgaagggcc tctctacgtc ttctccccaa
      421 ctgaagaact aaggaagcgg tggattcacc agctcaaaaa cgtaatccgg tacaacagtg
      481 atctggttca gaaatatcac ccttgcttct ggatcgatgg gcagtatctc tgctgctctc
      541 agacagccaa aaatgctatg ggctgccaaa ttttggagaa caggaatgga agcttaaaac
      601 cggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      661 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      721 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      781 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      841 gctaccc
//
