LOCUS       sequence-81909-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Hammond GR, Machner MP, Balla T
  TITLE     A novel probe for phosphatidylinositol 4-phosphate reveals multiple 
            pools beyond the Golgi.
  JOURNAL   J Cell Biol. 2014 Apr 7.
  PUBMED    24711504
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2014 Apr 7."
COMMENT     Sequence Label: mCherry-FKBP-MTM1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgaa gcgcggccgc gggagcagga
      781 ggagcagctc gagcggcgct gggagtgcag gtggaaacca tctccc
//
