LOCUS       sequence-81932-e         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a bicistronic IRES-DsRed containing retroviral vector, MCS is 
            expanded from pMSCV-IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, 
            Vignali DA
  TITLE     Generation of T-cell receptor retrogenic mice.
  JOURNAL   Nat Protoc. 2006;1(1):406-17.
  PUBMED    17406263
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "406-17"
COMMENT     Sequence Label: pMSCV-IRES-DsRed FP (pMIDsRed II)
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(253..271)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(303..323)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
       61 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
      121 ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
      181 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      241 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      301 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
      361 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      421 aggacggcgg cgtggtgacc gtgacccagg actcctccct gca
//
