LOCUS       sequence-81967-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA 1 against rodent Rem2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Ghiretti AE, Paradis S
  TITLE     The GTPase Rem2 regulates synapse development and dendritic 
            morphology.
  JOURNAL   Dev Neurobiol. 2011 May;71(5):374-89. doi: 10.1002/dneu.20868.
  PUBMED    21485012
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dneu"; 
            journalName: "Dev Neurobiol"; date: "2011-05"; volume: "71"; issue: 
            "5"; pages: "374-89"
COMMENT     Sequence Label: pSuper-Rem2-1
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        78..292
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     213..232
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(365..381)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(410..428)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(449..465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(449..465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(462..484)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    473..489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(497..503,504..521,522..527))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    542..563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(680..697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaactc ccctttaagg
       61 ccgggctgca ggaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca
      121 gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg
      181 acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata
      241 aacgtgaaat gtctttggat ttgggaatct tataagttct gtatgagacc acagatcccc
      301 gacggatcat ggtggacaat tcaagagatt gtccaccatg atccgtcttt ttggaaaagc
      361 ttatcgatac cgtcgacctc gagggggggc ccggtaccag cttttgttcc ctttagtgag
      421 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      601 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      721 ga
//
