LOCUS       sequence-82012-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-HA3-TSC1
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagcatttag gtgacactat agaatagggc cctctagtct tagctgtgtt catgatgagt
       61 ctcattgtag tccatgatat gtagctgtcc aacactgtcc ggggtcgggg gagacgggtg
      121 agggccatct aggttcaggg gaatcttggc ttccacaccc aagtctttgc ccagttctgt
      181 ctttaggctc tcagaaaggc tactggtcat gccgtcctca tcacactggc tctcgctctt
      241 attacgaaat aactctcgag ccttcatacc caggaagctt tttgaactgg gaagtgagcc
      301 cacagtggtg gggatgctgg cagacgcttc tcccatagtc gtctcccacc gactgctgaa
      361 tgggcctgcc ctctggtgtg ggggtttctc tggggtagaa agctcgctgc tgctgctgct
      421 gctgcctcca ccacctctgc ttccactact gccccgggcg ctgctgggcc tgggggtctt
      481 ggtctcaccg ttgtggccag atgcctcttc attgtgccct accatggaat ctgagcaccc
      541 gtcattacaa cagtcaagcc tttcttctgc tgcttcagct gcttctgctt tttcttcttc
      601 aagttttttc aggaggccat ctttctccaa cctgccatat aaatctaaga tctccaattc
      661 aaacacctgg gttatccttt tctgagcctc atacctgctc tctgcggcct gcagctgtcc
      721 tcttgcctgg agtttgacat cctctagata tttcttctgt tccaaaagaa ggtggtcttt
      781 cttggccagg tgagattcca gttccaaaat ccgtttttgg gaggtatcaa gcctctgagt
      841 ctgctggaga acatggcttc tgtttttttc tagctctttc cgataggcgg ctttcatcat
      901 ttctacttcc tttgtggtat ctgagtgctt gttctgcagt tgttccaaat agagctcgtt
      961 gacctcccca agaaccaaca gctgcctgtt caagaactcc atctgctgct ggac
//
