LOCUS       sequence-82091-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=117nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_117/pcDNA3
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(320..397)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 aatagggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt
       61 cttactgctc gttcttcagc actctctcca cgaagctctt gatgtacttg cccatctcgt
      121 cgggggcgtc ctcctggagg aagtgcaggc ccttcacctt cacgaactcg gtgttgggga
      181 acttcttggc gccctccacg atggcgttgc tgaagaagcc ggggtcaccc tcgatgaaca
      241 gcttgggcag gtcgtcgctg gctctcaggt aggcgttgta gtttctcacg atctgcacca
      301 cgtcgggctt gccgagctcc agtgccccgg agctggagat cttcttgaac cggttggcag
      361 cgctgacggc aatgaagttt ttcttccagc gcctcttgga gccccccttt gctgtcatca
      421 tttgtacaaa ctcttcatag tttacttggc catcaccatc gatatctgct tcccttatca
      481 tttcatcaac ttcttcatct gttaacttct ccccgaggtt tgtcatgacg tgacgtaatt
      541 cagcagcgct gatgtagccg ttcccatcct tgtcaaaaac acggaatgct tctcggattt
      601 cctcttcgct gtctgtgtcc ttcatttttc tagccatcat agtaagaaat tcaggaaagt
      661 aaatcgttcc attgccatca gcatcgactt cattgatcat atcctgcaat tctgcttccg
      721 ttgggttttg tccaagcgac ctcataacgg tgccaagttc ctttgtggtg atggtgccgt
      781 ccccatcctt gtcgaataat gagaaggctt ctttgaactc tgcaatctgc tcttctgtca
      841 gttggtcatg catccatggg cccttcacca ggg
//
