LOCUS       sequence-82179-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral bicistronic expression of p18Ink4c and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Forget A, Ayrault O, den Besten W, Kuo ML, Sherr CJ, Roussel MF
  TITLE     Differential post-transcriptional regulation of two Ink4 proteins, 
            p18 Ink4c and p19 Ink4d.
  JOURNAL   Cell Cycle. 2008 Dec;7(23):3737-46. Epub 2008 Dec 13.
  PUBMED    19029828
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2008-12"; volume: "7(23)"; pages: "3737-46. Epub 
            2008 Dec 13"
COMMENT     Sequence Label: pMSCV-p18Ink4c-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(710..727)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cttctctagg cgccggaatt cgccaccatg gccgagcctt gggggaacga gttggcgtcc
       61 gcagctgcca ggggggacct agagcaactt actagtttgt tgcaaaataa tgtaaacgtc
      121 aacgctcaaa atggatttgg gagaactgcg ctgcaggtta tgaaacttgg aaatccggag
      181 attgccagga ggcttctcct cagaggtgct aatcccaatt tgaaagatgg aactggtttt
      241 gctgtcattc atgatgctgc cagagcaggt ttcctggaca ctgtacaggc tttgctggag
      301 ttccaggctg atgttaacat tgaagataat gaagggaacc tgcccttgca cttggctgcc
      361 aaggaaggcc acctccctgt ggtggagttc cttatgaagc acacagcctg caatgtgggg
      421 catcggaacc ataaggggga caccgccttc gacttggcca ggttctatgg aagaaatgag
      481 gtcattagcc tgatggaggc aaatggggtt gggggagcca caagcctgca gtgactcgag
      541 cgggatcaat tccgcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
      601 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
      661 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
      721 ggaatgcaag
//
