LOCUS       sequence-82185-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin gamma 1 antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgG1/lambda
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtgttgtga aaaccaccgc taattcaaag caaatggact ggacctggag gatcctcttc
       61 ttggtggcgg ccgccacagg cgcgcactcc gaattcgata tcctcgaggc tagcaccaag
      121 ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc
      181 ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc
      241 gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc
      301 ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac
      361 gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac
      421 aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc
      481 ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc
      541 gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc
      601 gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt
      661 gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc
      721 aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg
      781 cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac
      841 caggtcagcc tgacctgcct ggtcaaaggc t
//
