LOCUS       sequence-8223-ex       11419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL
  TITLE     Production and design of more effective avian 
            replication-incompetent retroviral vectors.
  JOURNAL   Dev Biol. 1999 Oct 15. 214(2):370-84.
  PUBMED    10525341
REFERENCE   2  (bases 1 to 11419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            1999 Oct 15. 214(2):370-84."
COMMENT     Sequence Label: pRISAP (CC#8)
FEATURES             Location/Qualifiers
     source          1..11419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1243..1831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1732..1751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1985..2002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2017..2157
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2083..2102)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2243..2265
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2259..2450)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        3295..3556
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             8843..10342
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIILVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLGERQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRHPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSFIGLAAGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     promoter        10584..10845
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1381 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1921 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1981 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     2041 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     2101 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     2161 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2221 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2281 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     2341 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     2401 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac ttgatgcctc
     2461 cgtgtaaggg ggaatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     2521 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     2581 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     2641 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     2701 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     2761 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     2821 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     2881 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     2941 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     3001 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     3061 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
     3121 gcgacgcaac gcggggaggc agacaaggta tagggcggcg atgcgatgta cgggccagat
     3181 atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa agcggggctt cggttgtacg
     3241 cggttaggag tccccttagg atatagtagt ttcgcttttg catagggagg gggaaatgta
     3301 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     3361 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     3421 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattccgcat
     3481 tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgccattt gaccattcac
     3541 cacattggtg tgcacctggg ttgatggccg gaccgttgat tccctgacga ctacgagcac
     3601 ctgcatgaag cagaaggctt catttggtga ccccgacgtg atagttaggg aatagtggtc
     3661 ggccacagac ggcgtggcga tcctgtctcc atccgtctcg tctatcggga ggcgagttcg
     3721 atgaccctgg tggagggggc tgcggcttag ggaggcagaa gctgagtacc gtcggaggga
     3781 gctccagggc ccggagcgac tgacccctgc cgagaactca gagggtcgtc ggaagacgga
     3841 gagtgagccc gacgaccacc ccaggcacgt ctttggtcgg cctgcggatc aagcatggaa
     3901 gccgtcatta aggtgatttc gtccgcgtgt aaaacctatt gcgggaaaat ctctccttct
     3961 aagaaggaaa taggggccat gttgtccctg ttacaaaagg aagggttgct tatgtctccc
     4021 tcagatttat attctccggg gtcctgggat cccatcactg cggcgctctc ccagcgggca
     4081 atggtacttg gaaaatcggg agagttaaaa acctggggat tggttttggg ggcattgaag
     4141 gcggctcgag aggaacaggt tacatctgag caagcaaagt tttggttggg attaggggga
     4201 gggagggtct ctcccccagg tccggagtgc atcgagaaac cagctacgga gcggcgaatc
     4261 gacaaagggg aggaggtggg agaaacaact gtgcagcgag atgcgaagat ggcgccagag
     4321 gaagcggcca cacctaaaac cgttggcaca tcctgctatc attgcggaac agctgttggc
     4381 tgcaattgcg ccaccgccac agcctcggcc cctcctcccc cttatgtggg gagtggtttg
     4441 tatccttccc tggcgggggt gggagagcag cagggccagg gagataacac gtctcggggg
     4501 gcggagcagc caagggagga gccagggcac gcgggtcagg cccctgggcc ggccctgact
     4561 gactgggcaa gggtaaggga ggagcttgcg agtactggtc cgcccgtggt ggccatgcct
     4621 gtagtgatta agacagaggg acccgcctgg acccctctgg agccaaaatt gatcacaaga
     4681 ctggctgata cggtcaggac caagggctta cgatccccga tcactatggc agaagtggaa
     4741 gcgctcatgt cctccccgtt gctgccgcat gacgtcacga atctaatgag agtgatttta
     4801 ggacctgccc catatgcctt atggatggac gcttggggag tccaactcca gacggttata
     4861 gcggcagcca ctcgcgaccc ccgacaccca gcgaacggtc aagggcgggg ggaacggact
     4921 aacttggatc gattaaaggg cttagctgat gggatggtgg gcaacccaca gggtcaggcc
     4981 gcattattaa gaccggggga attggttgct attacggcgt cggctctcca ggcgtttaga
     5041 gaagttgccc ggctggcgga acctgcaggt ccatgggcgg acatcacgca gggaccatct
     5101 gagtcctttg ttgattttgc caatcggctt ataaaggcgg ttgaggggtc agatctcccg
     5161 ccttccgcgc gggctccggt gatcattgac tgctttaggc agaagtcaca gccagatatt
     5221 cagcagctta tacgggcagc accctccacg ctgaccaccc caggagagat aatcaaatat
     5281 gtgctagaca ggcagaagat tgcccctctt acggatcaag gcatagccgc ggccatgtcg
     5341 tctgctatcc agcccttagt tatggcagta gtcaatagag agagggatgg acaaactggg
     5401 tcgggtggtc gtgcccgagg gctctgctac acttgtggat ccccgggaca ttatcaggca
     5461 cagtgcccga aaaaacgaaa gtcaggaaac agccgtgagc gatgtcagct gtgtgacggg
     5521 atgggacaca acgctaaaca gtgtaggaag cgggatggca accagggcca acgcccagga
     5581 agaggtctct cttcggggcc gtggcccggc cctgagcagc ctgccgtctc gttagcgatg
     5641 acaatggaac ataaagatcg ccccttggtt agggtcattc tgactaacac tgggagtcat
     5701 ccagtcaaac aacgttcggt gtatatcacc gcgctgttgg actccggagc ggacatcact
     5761 attatttcgg aggaggattg gcctactgat tggccggtgg tggacaccgc gaacccacag
     5821 atccatggca taggaggggg aattcccatg cgaaaatccc gggatatgat agagttgggg
     5881 gttattaacc gagacgggtc gttggagcga cccctgctcc tcttccccgc agtcgctatg
     5941 gttagaggga gtatcctagg aagagattgt ctgcagggcc tagggctccg cttgacaaat
     6001 ttatagggag ggccactgtt ctcactgttg cgctacatct ggctattccg ctcaaatgga
     6061 agccagaccg cacgcctgtg tggattgacc agtggcccct ccctgaaggt aaacttgtag
     6121 gcctaacgca attagtggaa aaagaattac agttaggaca tatagagccc tcacttagtt
     6181 gttggaacac acctgttttt gtgatccgga aggcttccgg gtcttatcgc ttattgcatg
     6241 atttgcgcgc tgttaacgcc aagcttgtcc cttttggggc cgtccaacag ggggcgccag
     6301 ttctctccgc gctcccgcgt ggctggcccc tgatggtcct agacctcaag gattgcttct
     6361 tttctatccc tcttgcggaa caagatcgcg aagcttttgc atttacgctc ccctctgtga
     6421 ataaccaggc ccccgctcga agattccaat ggaaggtctt gccccaaggg atgacctgtt
     6481 ctcccactat ctgtcagttg gtagtgggtc aggtgctcga gcccttgcga ctcaagcacc
     6541 cagctctgtg catgttgcct tatatggacg atcttttgct agccgcctca agtcatgatg
     6601 ggttggaagc ggcagggaag gaggttatca gtacattgga aagagccggg ttcactattt
     6661 cgccggataa gatccagagg gagcccggag tacaatatct tgggtacaag ttaggcagta
     6721 cgtatgtagc acccgtaggc ctggtagcag aacccaggat agccaccttg tgggatgttc
     6781 aaaagctggt ggggtcactt cagtggcttc gcccagcgtt agggatcccg ccacgactga
     6841 tgggtccctt ttatgagcag ttacgagggt cagatcctaa cgaggcgagg gaatggaatc
     6901 tagacatgaa aatggcctgg agagagatcg tacagcttag cactactgct gccttggaac
     6961 gatgggaccc tgcccagcct ctggaaggag cggtcgctag atgtgaacag ggggcaatag
     7021 gggtcctggg acagggactg tccacacacc caaggccatg tttgtggtta ttctccaccc
     7081 aacccaccaa ggcgtttact gcttggttag aagtgctcac ccttttgatt actaagctac
     7141 gcgcttcggc agtgcgaacc tttggcaagg aggttgatat cctcctgttg cctgcatgct
     7201 tccgggagga ccttccgctc ccggagggga tcctgttagc acttaggggg tttgcaggaa
     7261 aaatcaggag tagtgacacg ccatctattt ttgacattgc gcgtccactg catgtttctc
     7321 tgaaagtgag ggttaccgac caccctgtgc cgggacccac tgtctttacc gacgcctcct
     7381 caagcaccca taaaggggtg gtagtctgga gggagggccc aaggtgggag ataaaagaaa
     7441 tagttgattt gggggcaagt gtacaacaac tggaggcacg cgctgtggcc atggcacttc
     7501 tgctgtggcc gacaacgccc actaatgtag tgactgactc tgcgtttgtt gcgaaaatgt
     7561 tactcaagat gggacaggag ggagtcccgt ctacagcggc ggcttttatt ttagaggatg
     7621 cgttaagcca aaggtcagcc atggccgccg ttctccacgt gcggagtcat tctgaagtgc
     7681 cagggttttt cacagaagga aatgacgtgg cagatagcca agccaccttt caagcgtatc
     7741 ccttgagaga ggctaaagat cttcataccg ctctccatat tggaccccgc gcgctatcca
     7801 aagcgtgtaa tatatctatg cagcaggcta gggaggttgt tcagacctgc ccgcattgta
     7861 attcagcccc tgcgttggag gccggggtaa accctagggg tttgggaccc ctacagatat
     7921 ggcagacaga ctttacgctt gagcctagaa tggctccccg ttcctggctc gctgttactg
     7981 tggacaccgc ctcatcagcg atagtcgtaa ctcagcatgg ccgtgttaca tcggttgctg
     8041 cacaacatca ttgggccacg gctatcgccg ttttgggaag accaaaggcc ataaaaacag
     8101 ataacgggtc ctgcttcacg tccagatcca cgcgagagtg gctcgcgaga tgggggatag
     8161 cacacaccac cgggattccg ggaaattccc agggtcaagc tatggtagag cgggccaacc
     8221 ggctcctgaa agataggatc cgtgtgctcg cggaggggga cggctttatg aaaagaatcc
     8281 ccaccagcaa acagggggaa ctattagcca aggcaatgta tgccctcaat cactttgagc
     8341 gtggtgaaaa cacaaaaaca ccgatacaaa aacactggag acctaccgtt cttacagaag
     8401 gacccccggt taaaatacga atagagacag gggagtggga aaaaggatgg aacgtgctgg
     8461 tctggggacg aggttatgcc gctgtgaaaa acagggacac tgataaggtt atttgggtac
     8521 cctctcggaa agttaaaccg gatgtcaccc aaaaggatga ggtgactaag aaagatgagg
     8581 cgagccctct ttttgcaggc atttctgact ggataccctg ggaagacgag caagaaggac
     8641 tccaaggaga aaccgctagc aacaagcaag aaagacccgg agaagacacc cttgctgcca
     8701 acgagagtta attatcgatc aaggcaggaa agacagctat tggtaattgt gaaatacgct
     8761 tttgtctgtg tgctgcagga gctgagctga ctctgctggt ggcctcgcgt accactgtgg
     8821 cgtcgacaat atggggtcga ggctgctgct gctgctgctg ctgggcctga ggctacagct
     8881 ctccctgggc atcatcctag ttgaggagga gaacccggac ttctggaacc gcgaggcagc
     8941 cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat
     9001 catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg
     9061 gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt
     9121 ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac
     9181 ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg
     9241 ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa
     9301 gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc
     9361 cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc
     9421 ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga
     9481 tgtgatccta ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta
     9541 cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg
     9601 gctcggcgaa cgccagggtg cccggtacgt gtggaaccgc actgagctca tgcaggcttc
     9661 cctggacccg tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga
     9721 gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg
     9781 cctgctgagc agacaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca
     9841 tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc
     9901 cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga
     9961 ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcct tcatcgggct
    10021 ggccgctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc
    10081 aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc
    10141 cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt
    10201 ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt
    10261 catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc
    10321 gccccccgcc ggcaccaccg acgccgcgca cccggggcgg tccgtggtcc ccgcgttgct
    10381 tcctctgctg gccgggaccc tgctgctgct ggagacggcc actgctccct gactatgata
    10441 tcgatactag tccgatgtac gggccagata tacgcgtatc tgaggggact agggtgtgtt
    10501 taggcgaaaa gcggggcttc ggttgtacgc ggttaggagt ccccttagga tatagtagtt
    10561 tcgcttttgc atagggaggg ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac
    10621 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
    10681 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
    10741 tggattggac gaaccactga attccgcatt gcagagatat tgtatttaag tgcctagctc
    10801 gatacaataa acgccatttg accattcacc acattggtgt gcacctgggt tgatggccgg
    10861 accgttgatt ccctgacgac tacgagcacc tgcatgaagc agaaggcttc atttggtgac
    10921 cccgacgtga tagttaggga atagtggtcg gccacagacg gcgtggcgat cctgtctcca
    10981 tccgtctcgt ctatcgggag gcgagttcga tgaccctggt ggagggggct gcggcttagg
    11041 gaggcagaag ctgagtaccg tcggagggga tccacaggac gggtgtggtc gccatgatcg
    11101 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt
    11161 cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca
    11221 gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca
    11281 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
    11341 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
    11401 aactaccgca ttaaagctt
//
