LOCUS       sequence-82275-e        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] 
            (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is 
            cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagcatcg agatccccgc ggggctcacg gagctgctgc agggcttcac ggtggaggtg
       61 ctgaggcacc agcccgccga cctgctggag ttcgcgctgc agcacttcac gcggctgcag
      121 caggagaacg agcgcaaggg cgccgcgcgc ttcggccatg agggcaggac ctggggggac
      181 gcgggcgcag ccgccggggg cggaaccccc agtaagggtg tcaacttcgc ggaggagccc
      241 atgcgctccg attccgagaa cggcgaagag gaggaggccg cggaagcagg ggcgttcaac
      301 gctccagtta taaaccggtt cacaaggcgt gcctcggtat gtgcagaagc ttataatcct
      361 gatgaagaag aagatgatgc agagtccagg ataatacatc ccaaaactga cgatcaaaga
      421 aacagattgc aagaagcctg caaagacatc ctgctgttta agaacctgga tccagaacag
      481 atgtctcaag tattagatgc catgtttgaa aaattggtca aagaagggga acacgtaatc
      541 gatcaaggtg atgatggtga caacttttac gtcatcgaca gaggaacatt tgatatttat
      601 gtaaaatgtg atggcgttgg aagatgcgtt ggtaactatg acaatcgtgg gagttttgga
      661 gaactggcct taatgtacaa tacacccaga gcagctacaa tcactgctac ctctcctggt
      721 gctctgtggg gtttggacag ggtgaccttc aggagaataa tagtaaaaaa caatgccaaa
      781 aagaggaaga tgtacgagag ctttattgag tcactgccat tcctcaagtc tctggaggtt
      841 tctgaacgcc tgaaggtggt agatgtgatt ggcaccaaag tttacaacga tggagaacag
      901 atcattgctc agggagactc ggcggattcg ttcttcattg tagaatctgg agaagtgaga
      961 attactatga agagaaaggg taaatcagac atcgaagaga acggtgctgt ggaaatcgct
     1021 cggtgtctcc ggggacagta ttttggagag cttgccctgg tcactaacaa gccaagagca
     1081 gcatctgcac acgccattgg gactgtcaaa tgcttagcca tggatgtgca agcatttgag
     1141 aggcttctgg gaccttgcat ggaaattatg aagaggaata tcgccaccta tgaggagcaa
     1201 ttagttgcac tgtttgggac aaacatggat attgttgagc ccactgca
//
