LOCUS       sequence-8234-ex        7480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pCAG-NL1AB
FEATURES             Location/Qualifiers
     source          1..7480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1910..1936
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2392..2401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4362..4371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4484..4503)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4549..4604
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4603..4622)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4965..4981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4965..4981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4978..5000)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4989..5005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5013..5019,5020..5037,5038..5043))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5058..5079
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5138..5333
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5184..5319
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5339..5473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5376..5395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5430..5449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5541..5558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5712..6300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5792..5811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6471..7262,7263..7331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7094..7113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7332..7436)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7467..7480,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc gctaccggac
     1741 tcagatctcg agctcaaagc ttgggaccat ggcacttccc agatgcatgt ggccaaatta
     1801 tgtttggaga gctatgatgg catgtgtggt ccacagggga tccggtgccc cattgactct
     1861 ctgcttgttg ggatgtttgc tacagacttt tcacgtactc tctcaaaagt atccatacga
     1921 cgttccggac tacgcattgg atgatgtaga cccattggtt actactaact ttggcaagat
     1981 taggggaatt aagaaagaac tcaataatga aattttgggt cctgtcattc agtttcttgg
     2041 ggttccatat gccgctccac caacaggaga acatcgtttc cagcctccag aaccaccatc
     2101 tccctggtct gacatccgga acgccactca gtttgctcct gtatgtcccc agaatatcat
     2161 tgatggcaga ttgcctgaag ttatgcttcc tgtgtggttc actaataact tggatgtggt
     2221 ttcatcatac gtccaagacc agagtgaaga ctgtctatac ttaaacatct atgtcccaac
     2281 tgaagatggt ccccttacaa agaaacacac agatgattta ggtgataatg acggtgctga
     2341 agatgaagat attcgggaca gtgggggtcc caaaccagtg atggtgtaca tccatggcgg
     2401 ctcttacatg gaaggtactg gaaatctgta tgatgggagt gtcttggcaa gctatggcaa
     2461 tgtgatcgtc atcacagtca actatcggct tggggtactt ggcttcttga gcacagggga
     2521 tcaggctgcc aaaggaaact acgggctcct tgacctcatc caggccctaa gatggaccag
     2581 cgagaacatt gggttctttg gtggtgaccc cttgcgaatc actgtgtttg gatcaggcgc
     2641 tgggggttca tgtgtcaacc tgctgacttt atcccattat tctgaaggta accgttggag
     2701 caattcaacc aaaggacttt ttcaacgagc aatagctcag agtggaacag ccctttccag
     2761 ctgggctgtt agtttccagc ctgcaaaata cgctagaatt ctggccacaa aagttggctg
     2821 caatgtttca gatacagtag agttagtaga atgcctgcag aagaagcctt acaaagaact
     2881 tgttgatcaa gatgttcaac cagcccgata ccacatagcc tttggacctg tgatcgatgg
     2941 tgatgtaata ccagatgacc ctcagatact gatggaacaa ggagagttcc tcaactatga
     3001 tataatgttg ggagttaacc aaggggaagg gttgaagttt gtcgaaaata tagtagatag
     3061 tgatgatggt gtatcagcca gtgatttcga ctttgctgtt tctaattttg ttgataattt
     3121 atatggatat cctgaaggca aagatgtttt gagagaaacc attaaattca tgtatactga
     3181 ctgggctgat cgccataatc ctgaaactag aaggaagaca ttgttggctt tgtttacgga
     3241 ccatcaatgg gtagcacctg ctgtggccac agcagacctt cactcgaact ttggctcacc
     3301 tacatacttc tatgcctttt atcatcattg ccaaacagat caagttccag cttgggctga
     3361 tgcagctcat ggggatgagg ttccctatgt gttgggaatc cccatgattg gccctacaga
     3421 gttatttcct tgcaatttct ccaagaatga tgtgatgttg agtgcagtag taatgacata
     3481 ctggacgaat tttgctaaaa ctggtgaccc aaatcaacca gttcctcaag acacaaaatt
     3541 catccatacc aaacccaacc gctttgaaga agtagcatgg accagatatt cccagaaaga
     3601 ccagctttat ctccatattg gattaaaacc gagagttaaa gagcattaca gagccaataa
     3661 ggtaaatctc tggctggagc tggtacctca tctgcataat ctcaatgaca tttctcagta
     3721 tacctcgaca acaactaaag tgccatccac ggacatcact ctcagaccta caaggaagaa
     3781 ttccacacca gtcacatcag cctttcccac tgccaaacag gatgatccca agcaacaacc
     3841 aagccccttc tcggtggatc agagggacta ctccacagag ctaagtgtca ctatcgcagt
     3901 gggggcctct ctgctgtttc tcaacatctt ggcttttgca gccctgtact acaagaagga
     3961 taagaggaga catgatgtcc accggaggtg cagccctcag cgcacgacca ccaacgacct
     4021 aacccatgct ccagaagagg aaattatgtc tctccaaatg aagcacactg acttggatca
     4081 cgagtgtgag tccatccatc cacatgaggt ggttcttcgg accgcctgtc ccccagatta
     4141 tactctagct atgaggaggt cacctgatga tattccacta atgacaccta acaccatcac
     4201 aatgattccc aacactatac cagggattca gcccttacat acattcaaca catttactgg
     4261 aggacagaat aatacactgc cccatcccca cccacacccc cattcacatt caacaaccag
     4321 ggtatagcca gatgagagag acaaactgat taatcccgcg gccatggcgg ccgggagcat
     4381 gcgacgtcgg gcccggggag ctcttcactc ctcaggtgca ggctgcctat cagaaggtgg
     4441 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     4501 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     4561 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     4621 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     4681 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     4741 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     4801 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     4861 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     4921 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     4981 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5041 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5101 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     5161 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5221 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5281 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     5341 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5401 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5461 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5521 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5581 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5641 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5701 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5761 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5821 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5881 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5941 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6001 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6061 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6121 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6181 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6241 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7381 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7441 tttccccgaa aagtgccacc tgggtcgaca ttgattattg
//
