LOCUS       sequence-82360-e         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial transcription factor with VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1-VP64
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttgccac catggacccc
      121 attcgtccgc gcaggccaag tcctgcccgc gagcttctgc ccggacccca accggatagg
      181 gttcagccga ctgcagatcg tggggtgtct gcgcctgctg gcagccctct ggatggcttg
      241 cccgctcggc ggacggtgtc ccggacccgg ctgccatctc cccctgcgcc ctcacctgcg
      301 ttctcggcgg gcagcttcag cgatctgctc cgtccgttcg atccgtcgct tcttgataca
      361 tcgcttcttg attcgatgcc tgccgtcggc acgccgcata cagcggctgc cccagcagag
      421 tgggatgagg cgcaatcggc tctgcgtgca gccgatgacc cgccacccac cgtgcgtgtc
      481 gctgtcactg ccgcgcggcc gccgcgcgcc aagccggccc cgcgacggcg tgctgcgcaa
      541 ccctccgacg cttc
//
