LOCUS       sequence-82401-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the endoplasmic reticulum (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS
  TITLE     A rapidly maturing far-red derivative of DsRed-Express2 for 
            whole-cell labeling.
  JOURNAL   Biochemistry. 2009 Sep 8;48(35):8279-81.
  PUBMED    19658435
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2009-09-8"; volume: "48(35)"; pages: 
            "8279-81"
COMMENT     Sequence Label: pEF.myc.ER-E2-Crimson
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     join(29..73,156..167)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(29..74,157..167)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             189..863
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     587..606
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             873..902
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(956..973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    962..1073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tttcaggtgt cgtgaacacg tggccaccat gggatggagc tgtatcatcc tcttcttggt
       61 agcaacagct acaggtaagg ggttaacagt agcaggcttg aggtctggac atatatatgg
      121 gtgacaatga catccacttt gcctttctct ccacaggcgc gcactcccag gtccaactgc
      181 aggtcgacat ggatagcact gagaacgtca tcaagccctt catgcgcttc aaggtgcaca
      241 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgtgggcgag ggcaagccct
      301 acgagggcac ccagaccgcc aagctgcaag tgaccaaggg cggccccctg cccttcgcct
      361 gggacatcct gtccccccag ttcttctacg gctccaaggc gtacatcaag caccccgccg
      421 acatccccga ctacctcaag cagtccttcc ccgagggctt caagtgggag cgcgtgatga
      481 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcaccc
      541 tcatctacca cgtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga
      601 agaagactct gggctgggag ccctccactg agcgcaacta cccccgcgac ggcgtgctga
      661 agggcgagaa ccacatggcg ctgaagctga agggcggcgg ccactacctg tgtgagttca
      721 agtccatcta catggccaag aagcccgtga agctgcccgg ctaccactac gtggactaca
      781 agctcgacat cacctcccac aacgaggact acaccgtggt ggagcagtac gagcgcgccg
      841 aggcccgcca ccacctgttc caggcggccg cagaacaaaa actcatctca gaagaggatc
      901 tgaatggggc cgcaagcgag aaggacgagc tgtagtctag aagctcgctg atcagcctcg
      961 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     1021 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     1081 ctgagt
//
