LOCUS       sequence-82410-e         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attttccgcc tgctcacgta tgggtttatc cctgtgcttg ttgtatgcgc ttaatacttt
       61 gtccagattg gcatcagcta ggatgactct cttactgaat tccgaaattt gctctatgat
      121 ttcgtcgaga taatgtttgt gctgctcaac aaaaagttgc ttctgttcgt tatcttcagg
      181 tgaacctttc aacttctcgt aatgggacgc taaatacagg aaattcacgt atttagacgg
      241 tagtgcgagt tcgttcccct tttgaagctc tccggcgcta gccaacatcc gttttcggcc
      301 attttctaac tcaaacagac tatactttgg tagtttaatt atgagatcct tttttacttc
      361 cttgtaacct ttcgcctcaa ggaagtcgat ggggttcttt tcaaaagacg agcgctccat
      421 aatcgttatc cccaataatt ctttgactga cttcagtttc ttggattttc ccttctcaac
      481 ttttgccact actaggacag aataggcaac tgtagggcta tcgaagccac cgtacttttt
      541 cgggtcccag tcctttttac gagcgatgag cttatcacta ttcctttttg gaagaatcga
      601 tt
//
