LOCUS       sequence-82412-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tagccgtcgg gatttagaga ggcgggcgct aagaatagcc ttcgcatcca cgccacttgc
       61 atttataggg ttctcttcaa acaactgatt ataggtttgt actaactgga tgaacagttt
      121 gtcgacatcc gagttgtccg gatttagatc accctcaatg agaaagtgcc cacggaactt
      181 tatcatatgg gcaagagcca agtagattaa cctcaggtcc gctttatcag ttgagtcaac
      241 tagctttttt ctgaggtgat aaatcgttgg gtacttttca tgatatgcca cctcatctac
      301 tatgtttcca aagatggggt gccgttcatg tttcttgtcc tcttcgacaa ggaaggactc
      361 ttccaaacgg tgaaagaaag aatcgtcaac tttggccatc tcattgctaa aaatttcttg
      421 taagtaacat attcggttct tgcgacgtgt ataccttctc cgagcggttc gtttcaggcg
      481 agtcgcctct gccgtttcgc cactatcgaa taggagggca ccgataagat tctttttaat
      541 cgaatgacgg tctgtgttcc ccaacacctt aaatttcttt gaaggtactt tgtattcatc
      601 ggttatgaca gcccatccaa cggaattagt gccgatagct aaaccaatag aatacttttt
      661 atcactttcg ggtgtggcgg actctgaggt cccgggagtc tcgctgccgc taaagtttat
      721 ctcgccgtta ttaaatttcc gtctgacttc ct
//
