LOCUS       sequence-82425-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Broad-host-range cre-lox system for antibiotic marker recycling in 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 2002 Nov;33(5):1062-7.
  PUBMED    12449384
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2002-11"; volume: "33(5)"; pages: "1062-7"
COMMENT     Sequence Label: pCM184
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        124..228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             413..791
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(447..466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtctagataa cttcgtataa tgtatgctat acgaagttat gcggccgcca tatgcatcca
       61 tggtaccaat tgtacagctg aattcagatc tagacgtcag gtggcacttt tcggggaaat
      121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
      781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      901 gctggctggt ttattgctga taaatctgg
//
