LOCUS       sequence-82496-e         272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null TD Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-TDcasN-
FEATURES             Location/Qualifiers
     source          1..272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
       61 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgag gggccgcaag
      121 atccggccac gatgacccgg tcgtcggttc agggcagggt accaggcacg cctaaccgtc
      181 agtgagattg gatgagtgaa cgatattgat cgagaagagc cctgcgcagc cgctgccgtg
      241 cctgcaggaa gcaacggccc ggagggtggc gg
//
