LOCUS       sequence-82601-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP tagged MeCP2-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
  TITLE     An AT-hook domain in MeCP2 determines the clinical course of Rett 
            syndrome and related disorders.
  JOURNAL   Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
  PUBMED    23452848
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "984-96"
COMMENT     Sequence Label: pDEST-hMeCP2-GFP
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(426..446)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
       61 accactttgt acaagaaagt tgggctaact ctctcggtca cgggcgtccg gctgtccaca
      121 ggctcctctc tgtttggcct tggcatggag gatgaaacaa tgtctttgcg ctctccctcc
      181 cctcggtgtt tgtacttttc tgcggccgtg gcggcggtgg caaccgcggg ctgagtctta
      241 gctggctcct tggggcagcc gtcgctctcc agtgagcctc ctctgggcat cttctcctct
      301 ttgcagacgc tgctgctcaa gtcctggggc tcaggggggc tggtggggtc ctcggagctc
      361 tcgggctcag gtggaggtgg gggcaggggt gggagcagtg gcacgggggc ctttggggac
      421 tctgagtggt ggtgatggtg gtggtgctcc ttcttggggg gtgaggaggc gctgctgctg
      481 cgccccttgg ggctgctctc cttgcttttc cgcccagggc tcttacaggt cttcagtcct
      541 ttcccgctct tctcaccgag ggtggacacc agcaggggct tcaccacttc cttgacctcg
      601 atgctgaccg tctcccgggt cttgcgcttc ttgatgggga gtacggtctc ctgcacagat
      661 cggatagaag actccttcac ggctttcttt ttggcctcgg cggcagcggc tgccaccaca
      721 ctccccggct ttcggccccg tttcttggga atggcctgag ggtcggcctc agcttttcgc
      781 ttcctgccgg ggcgtttgat caccatgacc tgggtggatg tggtggcccc acccccctca
      841 gccttgcccc ctggcgaagt ttgaaaaggc atcttgacaa ggagcttccc aggacttttc
      901 tccaggaccc
//
