LOCUS       sequence-82603-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Mxe intein/chitin binding domain tagged 
            MeCP2-R270X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
  TITLE     An AT-hook domain in MeCP2 determines the clinical course of Rett 
            syndrome and related disorders.
  JOURNAL   Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
  PUBMED    23452848
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "984-96"
COMMENT     Sequence Label: pTXB1-hMeCP2-R270X
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac atatggtagc tgggatgtta
       61 gggctcaggg aagaaaagtc agaagaccag gacctccagg gcctcaagga caaacccctc
      121 aagtttaaaa aggtgaagaa agataagaaa gaagagaaag agggcaagca tgagcccgtg
      181 cagccatcag cccaccactc tgctgagccc gcagaggcag gcaaagcaga gacatcagaa
      241 gggtcaggct ccgccccggc tgtgccggaa gcttctgcct cccccaaaca gcggcgctcc
      301 atcatccgtg accggggacc catgtatgat gaccccaccc tgcctgaagg ctggacacgg
      361 aagcttaagc aaaggaaatc tggccgctct gctgggaagt atgatgtgta tttgatcaat
      421 ccccagggaa aagcctttcg ctctaaagtg gagttgattg cgtacttcga aaaggtaggc
      481 gacacatccc tggaccctaa tgattttgac ttcacggtaa ctgggagagg gagcccctcc
      541 cggcgagagc agaaaccacc taagaagccc aaatctccca aagctccagg aactggcaga
      601 ggccggggac gccccaaagg gagcggcacc acgagaccca aggcggccac gtcagagggt
      661 gtgcaggtga aaagggtcct ggagaaaagt cctgggaagc tccttgtcaa gatgcctttt
      721 caaacttcgc cagggggcaa ggctgagggg ggtggggcca ccacatccac ccaggtcatg
      781 gtgatcaaac gccccggcag gaagcgaaaa gctgaggccg accctcaggc cattcccaag
      841 aaacggggcg cgtgcatcac gggagatgca ctagttgccc tacccgaggg cgagtcggta
      901 cgcatcgccg acatcgtgcc gggtgcgcgg cccaacagtg acaacgccat cgacctgaaa
      961 gtccttgacc ggcatgg
//
