LOCUS       sequence-82648-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serves as PCR-template to generate HR donors for C-terminal YFP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB20
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             334..732
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(781..814)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acgagctgta caagtaaact agtgaagttc ctatactttc tagagaatag gaacttcaaa
       61 taaacatatg ctgttggaat atactattca acctacaaaa ataacgttaa acaacactac
      121 tttatatttg atatgaatgg ccacaccttt tatgccataa aacatattgt aagagaatac
      181 cactcttttt attccttctt tccttcttgt acgttttttg ctgtgagtag gtcgtggtgc
      241 tggtgttgca gttgaaataa cttaaaatat aaatcataaa actcaaacat aaacttgact
      301 atttatttat ttattaagaa aggaggtacc ggtatggcca agcctttgtc tcaagaagaa
      361 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
      421 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
      481 cattttactg ggggaccttg cgcagaactc gtggtgctgg gcactgctgc tgctgcggca
      541 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
      601 tgcggacggt gccgacaggt tcttctcgat ctgcatcctg ggatcaaagc catagtgaag
      661 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
      721 tgggagggct agaaattatt aaaaataaaa ctttaaaaat aaatttgttt ctaacatatg
      781 gaagttccta tactttctag agaataggaa cttcatcttt ctagaagatc tcctacaata
      841 ttctcagctg ccatggaaa
//
