LOCUS       sequence-82681-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses hM4Dnrxn and a red fluorescent label.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: CAG:: mCherry-2a-hM4Dnrxn
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..50)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          33..165
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(379..405)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(406..432)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac acctgtggag agaaaggcaa agtggatgtc
       61 agtaagacca ataggtgcct atcagaaacg caagagtctt ctctgtctcg acaagcccag
      121 tttctattgg tctccttaaa cctgtcttgt aaccttgata cttacctgcg gcctcgacct
      181 tagctattca tcggtacctc gagcaccggt tcacacatag tactctttgt ccttattctt
      241 cttgttctta ttcgctgact tcgccgatga gggctgcttt tccttcacca ccgctccgtt
      301 tgactgggcg gagttcgaga tatagttccg tgactcgtcc acatgatacg agccttcgtc
      361 ccggttccga tacttgtaag cgtaatccgg gacgtcatag ggataggcgt aatcggggac
      421 atcatatggg tatctcgctg tcccgatatt tcggtactgg cacaacagga gatggcggaa
      481 cgttttcttg aacgtggcgt tacacaatgc gtagcatgcc ggattgatag tggagttgac
      541 gtagcagagc caatacccga tcgaccagac cgtgtctggg atgcacgatt gacaaaatgt
      601 attcaccaac accatgacat tatacggagt ccacgtcaag ataaacgcca ggaggatggc
      661 gaaaatcgtt cttgtcacct ttctctctct cgcggccatt tgtcttttct tccgcacctg
      721 attccgcgcg attgacgcga actttctggc gacgttggcg gctggcctca taccagcggg
      781 cgttgcgggt acgatctcga tggctgttac gcactcatta cccgttt
//
