LOCUS       sequence-8271-ex         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  TITLE     Ristow lab plasmids
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBlue-hFX-HA(AscI)
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             645..671
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggcgcgccac catgggatgg actctcgggc gccgcgcagt agccggcctc ctggcgtcac
       61 ccagcccggc ccaggcccag accctcaccc gggtcccgcg gccggcagag ttggccccac
      121 tctgcggccg ccgtggcctg cgcaccgaca tcgatgcgac ctgcacgccc cgccgcgcaa
      181 gttcgaacca acgtggcctc aaccagattt ggaatgtcaa aaagcagagt gtctatttga
      241 tgaatttgag gaaatctgga actttgggcc acccaggctc tctagatgag accacctatg
      301 aaagactagc agaggaaacg ctggactctt tagcagagtt ttttgaagac cttgcagaca
      361 agccatacac gtttgaggac tatgatgtct cctttgggag tggtgtctta actgtcaaac
      421 tgggtggaga tctaggaacc tatgtgatca acaagcagac gccaaacaag caaatctggc
      481 tatcttctcc atccagtgga cctaagcgtt atgactggac tgggaaaaac tgggtgtact
      541 cccacgacgg cgtgtccctc catgagctgc tggccgcaga gctcactaaa gccttaaaaa
      601 ccaaactgga cttgtcttcc ttggcctatt ccggaaaaga tgcttaccct tacgacgtac
      661 cagactacgc agacacgtac cgatacatct aggcgcgcc
//
