LOCUS       sequence-82716-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Blasticidin 
            selection, V5 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX304
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..138
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(163..168,169..186,187..193)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(313..332)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 actgtcggga tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
       61 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
      121 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
      181 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      241 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      301 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      361 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      421 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      481 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      541 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      601 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      661 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
      721 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
      781 caa
//
