LOCUS       sequence-82753-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Coexpression plasmid of QuasAr2 and CheRiff for all-optical 
            electrophysiology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou
            P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, 
            Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, 
            Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
  TITLE     All-optical electrophysiology in mammalian neurons using engineered 
            microbial rhodopsins.
  JOURNAL   Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 
            2014 Jun 22.
  PUBMED    24952910
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3000"; journalName: "Nat Methods"; date: "2014-08"; 
            volume: "11"; issue: "8"; pages: "825-33"
COMMENT     Sequence Label: DRH296: FCK-Optopatch2
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..807)
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
ORIGIN
        1 cttgatgatg gccatgttat actcctcgcc cttgctcacc atcaacgtat cggcaccggc
       61 actgggttct ggtgcctcgg tgtcgcccag aatagcccgg gatctcaaca ggataaagcc
      121 aaagccgacc ttggcagtca cgtccaacac cataaacagc agagtttcga tgcccaggcc
      181 caccacgcca gcgccctcag tgcctatgat ccacaggata gggtaagcgg tccacagcac
      241 caagaccaga gctgtcaggg tgttaaaggt agatgccacc tcggggcccc gctcctttgc
      301 agcagatcgc agggatgtag ccagaacata gagcaccact atcatgcaaa ttgtagagaa
      361 caaccaccaa ctgtatctgg ctatggccgt gtggctcaag gctccgatga ggccagtgac
      421 gatcatcagg gcgtccacac ccaccagggt gccgatggtc acccgatcca ccttagcgag
      481 aagggccagg tgcagcagca gaagtggggt ggtaaacagc cactgggcgt acctggcata
      541 atagatatcc aacatttcgc ccccgacgga cacctcagta agcccgatac caaagaacat
      601 agacagatat gcggcggatg cgattccgga caccaggata gtcacagcgt aatattcccg
      661 ggcatcctta tcggtgactc cccatccgcg gaccagaaag tagaaggttc caatcagcat
      721 cagcagagtg ccgatgccca gccacagggt ttcaggtctg ccgtcaccca gtaggtcgta
      781 accagcctgc agagcgatac ttaccatggt ggatcccccg ctgcccccag aactaggggc
      841 cactcgcctg cccgtgctcc tgagtgcaaa
//
