LOCUS       sequence-82773-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N 
            oligomerizes under violet light (390-400nm) and dissociates under 
            cyan light (490-500nm).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Zhou XX, Chung HK, Lam AJ, Lin MZ
  TITLE     Optical control of protein activity by fluorescent protein domains.
  JOURNAL   Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854.
  PUBMED    23139335
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2012-11-9- 9"; volume: "338"; issue: 
            "6108"; pages: "810-4"
COMMENT     Sequence Label: pcDNA3-mNeptune2-N
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..809
                     /codon_start=1
                     /product="far-red fluorescent protein with spectra similar 
                     to those of mNeptune but improved brightness"
                     /label=mNeptune2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTG
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttatg gtgtctaagg gcgaagagct gattaaggag aacatgcaca
      121 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
      181 aaggcaagcc ctacgagggc acccagaccg gcagaatcaa ggtggtcgag ggcggccctc
      241 tccccttcgc cttcgacatc ctggctacct gcttcatgta cggcagcaag accttcatca
      301 accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc ttcacatggg
      361 agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac accagcctcc
      421 aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca tccaacggcc
      481 ctgtgatgca gaagaaaaca ctcggctggg aggccagtac cgagaccctg taccccgctg
      541 acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc
      601 actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
      661 gcgtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg
      721 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
      781 aacttaatgg catggacgag ctgtacaagg gacttaaggg atccgaattc ggcatgagcg
      841 tgatcaagcc cgacatgaaa atcaagctg
//
