LOCUS       sequence-82840-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP tagged MeCP2-G273X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
  TITLE     An AT-hook domain in MeCP2 determines the clinical course of Rett 
            syndrome and related disorders.
  JOURNAL   Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
  PUBMED    23452848
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "984-96"
COMMENT     Sequence Label: pDEST-hMeCP2-GFP-G273X
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(871..895)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(927..946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(928..946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttctccttt gctagccatt ctagatcgaa ccactttgct actgctactg ctactcggct
       61 ttcggccccg tttcttggga atggcctgag ggtcggcctc agcttttcgc ttcctgccgg
      121 ggcgtttgat caccatgacc tgggtggatg tggtggcccc acccccctca gccttgcccc
      181 ctggcgaagt ttgaaaaggc atcttgacaa ggagcttccc aggacttttc tccaggaccc
      241 ttttcacctg cacaccctct gacgtggccg ccttgggtct cgtggtgccg ctccctttgg
      301 ggcgtccccg gcctctgcca gttcctggag ctttgggaga tttgggcttc ttaggtggtt
      361 tctgctctcg ccgggagggg ctccctctcc cagttaccgt gaagtcaaaa tcattagggt
      421 ccagggatgt gtcgcctacc ttttcgaagt acgcaatcaa ctccacttta gagcgaaagg
      481 cttttccctg gggattgatc aaatacacat catacttccc agcagagcgg ccagatttcc
      541 tttgcttaag cttccgtgtc cagccttcag gcagggtggg gtcatcatac atgggtcccc
      601 ggtcacggat gatggagcgc cgctgtttgg gggaggcaga agcttccggc acagccgggg
      661 cggagcctga cccttctgat gtctctgctt tgcctgcctc tgcgggctca gcagagtggt
      721 gggctgatgg ctgcacgggc tcatgcttgc cctctttctc ttctttctta tctttcttca
      781 cctttttaaa cttgaggggt ttgtccttga ggccctggag gtcctggtct tctgactttt
      841 cttccctgag ccctaacatc ccagctacca tgccaacttt tttgtacaaa cttgtttgat
      901 caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca
      961 gtaa
//
