LOCUS       sequence-82893-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Lim J, Crespo-Barreto J, Jafar-Nejad P, Bowman AB, Richman R, Hill 
            DE, Orr HT, Zoghbi HY
  TITLE     Opposing effects of polyglutamine expansion on native protein 
            complexes contribute to SCA1.
  JOURNAL   Nature. 2008 Apr 10. 452(7188):713-8.
  PUBMED    18337722
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Apr 10. 452(7188):713-8."
COMMENT     Sequence Label: pDEST27-GST-ATXN1 FL(84Q)-S776A
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..72
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(86..97)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgactcacta tagggagaga gctatgaacg tcgcatgcac gcgatcaacc actttgtaca
       61 agaaagttgg cttgcctaca ttagaccggc cttcaatgca aatcttaacc tcctgaggaa
      121 ttagagaagg cttaggaaga gtcaaaggtg gttcgtcttc tgacttctcc agtttgcggc
      181 tctctggcgc cgcccacctc ctcttcctcg ttgccgcggg cttgctgggt tctattttgg
      241 tgaggaaggg cgctgcaggc aatcccattt tctctggaaa cttcagttcg ccattctcag
      301 agagcatctg ggcactcccc tggttgattc cgttttcctg ctcggcatac ctgtgtctgc
      361 tgcccgccag gccgtcggcc tttgagtgct tcagcaggac gctggcggga tccacgggct
      421 ggcccttttt aacagagccg ttcttcaggt tcttgagggt aagcgagatg cagacatccc
      481 caactgagag tttggaacac ggcaaatcaa agagctggct ggttctctcc ggacagcagg
      541 atgaccagcc ctgtccaaac acaaaaaaag gatactctac caaaacttca acgctgacct
      601 gggctcggtg ctccccgacg gcgaactgta tcacggccac gcccgggcta tggctgtctt
      661 caatcctctc tacggtgctg gagtcgatct tcaggtcgtt gcttatctct gcactctgga
      721 tgaaatcttc tgtttttaag tcttccacct tctttagctc cccgttggcc aactggatga
      781 tggagccttt catgaagtag ggaggcagcg tagggggagc cgccgccggg gaggccacgg
      841 actgcaccac aggcaggtgg atctgggcct gcaccatggc tgggtaggcg gcctgggtga
      901 ccagggcctc a
//
