LOCUS       sequence-82949-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-based Calcium indicators.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Carlson HJ, Campbell RE
  TITLE     Circular permutated red fluorescent proteins and calcium ion 
            indicators based on mCherry.
  JOURNAL   Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 
            10.1093/protein/gzt052. Epub 2013 Oct 22.
  PUBMED    24151339
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt052"; journalName: "Protein Eng Des Sel"; date: 
            "2013-12"; volume: "26"; issue: "12"; pages: "763-72"
COMMENT     Sequence Label: CH-GECO2.1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(471..490)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     740..758
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat ccccatcgat
       61 gtctgctacc ctgatcattt catcaacctc ttcatctgtt aacttctctc caaggtttgt
      121 catcacgtgg cgaagctctg ctgcgccgat gtagccattg ccgtccttat caaacacacg
      181 gaacgcttct ctaatttcct cttcactgtc tgagtcattc atttttcttg ccatcatcgt
      241 caggaactca gggaagtcta atgtgccgtc accgtcggca tctacttcat tgatcatgtc
      301 ctgcagctct gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc ccagctcctt
      361 ggttgttatc gtcccagccc cgcccttgtc aaatagggag aaagcctctt taaattctgc
      421 gatctgctct tcagtcagtt ggtcacgcgt accgcccagc cagcccatgg tcttcttctg
      481 cattacgggg ccgttggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
      541 gccgtcctgc agggaggagt cctgggtcac ggtcaccaca cctccgtcct cgaagttcat
      601 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc cagtagtcgg ggatgtcggc
      661 tgggtgctta atgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
      721 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
      781 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttgacggagc cctccatgtg
      841 caccttgaag cgcatgaact ccttgatgaa ggccatgtta tcctccacgc ccttgctcac
      901 catggaaccg ccagtgccac ccttgtacag
//
