LOCUS       sequence-83022-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces
            ~10-fold higher titer virus. 3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiCRISPR v2
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      307..316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(335..546)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(608..683)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct
       61 cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca
      121 gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc aggttcttct
      181 tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact
      241 cgtcggtgat cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact
      301 tcttgtccat ggtggcagcg ctctagaacc ggtcctgtgt tctggcggca aacccgttgc
      361 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
      421 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
      481 caccggatca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
      541 ctgcccactg acgggcaccg gagccaattc ccactccttt caagacctag ctagcgaatt
      601 caaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      661 cttgctattt ctagctctaa aacagagacg tacaaaaaag agcaagaagc taaaaaagat
      721 ttaaaaatta tttttagcgc agttaatgga acaggaacta aatttacccc aaaaatatta
      781 cgtgaatcag gatataacgt tattgaggtt gaagagcatg catttgaaga tgaaacattt
      841 aaaaatgttg taaatccaaa tccagaattt gatcctgcat gaaaaatacc gcttgaatat
      901 ggtattaaac atgatgcaga tattattatt atgaatgacc cagatgctga cagatttgga
      961 atggcaataa aacatgatgg tcattttgta agattagatg gaaatcaaac aggaccaatt
     1021 t
//
