LOCUS       sequence-83046-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses I-SceI nuclease in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pKF257
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             310..330
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             349..375
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcggttacca tgatacttcg acgcataact gtagattttg gatagaatta aagagaaaat
       61 ggcgagagag taaaattccg gcgtcggcaa agtagagcaa aaaaatcagt ataccattta
      121 gctacctctc tcactcgcac gcagtgccgg ctcaagttgg gcgcggctct gcaattatcg
      181 attttcttgg ggtgtgtaac taatcatccg ttttcccttc ctcctcatcc acagcgtgaa
      241 ggtacctaat ccaaaatggg atcatcatca gacgacgaag ctacggccga tgcgcagcat
      301 gcagcaccac caaaaaaaaa acgaaaagta gaagacccac gatttatgta cccatacgat
      361 gttcctgact atgcgggtat gaaaaacatc aaaaaaaacc aggtaatgaa cctgggtccg
      421 aactctaaac tgctgaaaga atacaaatcc cagctgatcg aactgaacat cgaacagttc
      481 gaagcaggta tcggtctgat cctaggtgat gcttacatcc gttctcgtga tgaaggtaaa
      541 acctactgta tgcagttcga gtggaaaaac aaagcataca tggaccacgt atgtctgctg
      601 tacgatcagt gggtactgtc cccgccgcac aaaaaagaac gtgttaacca cctgggtaac
      661 ctggtaatca cctggggcgc ccagactttc aaacaccaag ctttcaacaa actggctaac
      721 ctgttcatcg ttaacaacaa aaaaaccatc ccgaacaacc tggttgaaaa ctacctgacc
      781 ccgatgtctc tggcatactg gttcatggat gatggtggta aatgggatta caacaaaaac
      841 tctaccaaca aatcgatcgt actgaacacc cagtctttca ctttcgaaga agtagaatac
      901 ctggtt
//
