LOCUS       sequence-83091-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mouse Oct3/4 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLPS-mOct4-gfp
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      48..142
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(178..213)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
       61 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
      121 ggcgtttttt attggtgaga atccaagcac tagtcatgac caggatctcc taggctagtt
      181 gtgattatga ttatgattat gattatgatt atgaccactt acctgggccc gcgcgccaaa
      241 cgaatggtct agaaagctcc tcgaggggtt tgaatgcatg gagagcccag agcagtgacg
      301 ggaacagagg gaaaggcctc gccctcagga aaagggactg agtagagtgt ggtgaagtgg
      361 gggcttccat agcctggggt gccaaagtgg ggacctgggg gcagaggaaa ggatacagcc
      421 ccccctggga aaggtgtccc tgtagcctca tactcttctc gttgggaata ctcaatactt
      481 gatcttttgc ccttctggcg ccggttacag aaccatactc gaaccacatc cttctctagc
      541 ccaagctgat tggcgatgtg agtgatctgc tgtagggagg gcttcgggca cttcagaaac
      601 atggtctcca gactccacct cacacggttc tcaatgctag ttcgctttct cttccgggcc
      661 tgcaccaggg tctccgattt gcatatctcc tgaaggttct cattgttgtc ggcttcctcc
      721 acccacttct ccagcagggg ccgcagctta cacatgttct taaggctgag ctgcaaggcc
      781 tcgaagcgac agatggtggt ctggctgaac acctttccaa agagaacgcc cagggtgagc
      841 cccacgtc
//
